State Key Laboratory of Pollution Control and Resource Reuse, Jiangsu Key Laboratory of Vehicle Emissions Control, School of the Environment, Nanjing University, Nanjing, Jiangsu, People's Republic of China.
School of Water Conservancy and Environment, University of Jinan, Jinan, Shandong, People's Republic of China.
J Toxicol Environ Health A. 2021 Jul 18;84(14):593-607. doi: 10.1080/15287394.2021.1919947. Epub 2021 May 5.
Animal bioassays have been developed to estimate oral relative bioavailability (RBA) of metals in soil, dust, or food for accurate health risk assessment. However, the comparability in RBA estimates from different labs remains largely unclear. Using 12 soil and soil-like standard reference materials (SRMs), this study investigated variability in lead (Pb) and arsenic (As) RBA estimates employing a mouse bioassay in 3 labs at Nanjing University, University of Jinan, and Shandong Normal University. Two performances of the bioassay at Nanjing University in 2019 and 2020 showed reproducible Pb and As RBA estimates, but increasing the number of mouse replicates in 2020 produced more precise RBA measurements. Although there were inter-lab variations in diet consumption rate and metal accumulation in mouse liver and kidneys following SRM ingestion due to differences in diet composition, bioassays at 3 labs in 2019 yielded overall similar Pb and As RBA estimates for the 12 SRMs with strong linear correlations between each 2 of the 3 labs for Pb (R = 0.95-0.98 and slope = 0.85-1.02) and As RBA outcomes (R = 0.46-0.86 and slope = 0.56-0.79). The consistency in RBA estimates was attributed to the relative nature of the final bioavailability outcome, which might overcome the inter-lab variation in diet consumption and metal uptake in mice. These results increased the confidence of use of mouse bioassays in bioavailability studies.
动物生物测定法已被开发出来，用于估计土壤、灰尘或食物中的金属的口服相对生物利用度（RBA），以进行准确的健康风险评估。然而，不同实验室得出的 RBA 估计值的可比性在很大程度上仍不清楚。本研究使用 12 种土壤和土壤标准参考物质（SRM），通过南京大学、济南大学和山东师范大学的 3 个实验室的小鼠生物测定法，研究了铅（Pb）和砷（As）RBA 估计值的变异性。南京大学 2019 年和 2020 年进行的两次生物测定实验结果表明，Pb 和 As RBA 估计值具有可重复性，但在 2020 年增加了小鼠重复次数，得出了更精确的 RBA 测量结果。尽管由于饮食组成的差异，3 个实验室在 SRM 摄入后小鼠的饮食消耗率和肝脏及肾脏中的金属积累存在差异，但 2019 年 3 个实验室的生物测定实验对 12 个 SRM 的 Pb 和 As RBA 估计值总体上是相似的，3 个实验室中的 2 个实验室之间的 Pb（R=0.95-0.98，斜率=0.85-1.02）和 As RBA 结果（R=0.46-0.86，斜率=0.56-0.79）之间存在很强的线性相关性。RBA 估计值的一致性归因于最终生物利用度结果的相对性质，这可能克服了饮食消耗和小鼠金属摄取的实验室间差异。这些结果增加了在生物利用度研究中使用小鼠生物测定法的信心。
