Department of Applied Chemistry, Graduate School of Engineering, Osaka Prefecture University.
PRESTO, Japan Science and Technology Agency.
Anal Sci. 2021 Jun 10;37(6):799-802. doi: 10.2116/analsci.21C003. Epub 2021 Apr 30.
Aptamers, single-stranded DNAs/RNAs with a strong and specific interaction towards a target molecule, have wide applications in the fields of medicine and biosensors. In conventional aptamer selection methods, it is difficult to obtain "preorganized" and/or "induced-fit" type of aptamers selectively. In this study, separation and fractionation of single-stranded DNAs with/without stable preorganized structures were carried out using capillary sieving electrophoresis. The fractionated DNAs showed different mobilities and thermodynamic stabilities of their secondary structures; this outcome is deemed to be necessary for the synthesis of novel aptasensors with a desirable sensing mechanism.
适体是一种与靶分子具有强特异性相互作用的单链 DNA/RNA，在医学和生物传感器领域有广泛的应用。在传统的适体筛选方法中，很难获得具有“预组织”和/或“诱导契合”型的适体。在本研究中，使用毛细管筛分电泳对具有/不具有稳定预组织结构的单链 DNA 进行了分离和分级。分级后的 DNA 显示出不同的迁移率和二级结构的热力学稳定性；这一结果对于合成具有理想传感机制的新型适体传感器是必要的。
