Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran.
Department of Endocrinology and Female Infertility, Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, Iran.
Sci Rep. 2021 May 5;11(1):9595. doi: 10.1038/s41598-021-87175-1.
This study aimed to investigate the relationship between follicular fluid Bisphenol A (BPA) concentrations with alterations of ICAM-1 and HLA-G genes and proteins expression as well as methylation profiles in the cumulus cells of poor ovarian response (POR) women based on their healthy lifestyle habit. Eighty women under the age of 35 were divided into two groups: 1-POR without using plastic containers (n = 40) and 2-POR with using plastic containers (n = 40). The ICAM-1 and HLA-G genes and protein expressions were examined by the quantitative PCR and western blotting technique. The methylation pattern was investigated by the methylation-specific PCR. Total BPA in follicular fluid was measured with high-performance liquid chromatography technique and the detection limit was 1.14 ng/ml. ICAM-1 and HLA-G genes were differentially expressed between the two groups studied. ICAM-1, HLA-G genes, and protein expressions in group 1 were up-regulated compared to the second group (P < 0.05). While DNA methylation status in group 1 were decreased compared to the other group (P < 0.05). The concentration of BPA in the follicular fluid of group 1 was lower compared to the second group (P < 0.05). The oocyte quality and clinical pregnancy ratio showed significantly higher in group 1 than in the other ones (P < 0.05). The alteration of ICAM-1 and HLA-G gene expressions in POR women is probably related to BPA concentration. As a result Lifestyle habits may also affect the methylation pattern and protein levels in the cumulus cells of POR women. Additionally, lifestyle habits may be considered as a marker for ovulation, oocyte maturation, preimplantation, and clinical pregnancy process.
本研究旨在探讨卵泡液中双酚 A（BPA）浓度与不良卵巢反应（POR）妇女卵丘细胞中细胞间黏附分子 1（ICAM-1）和人类白细胞抗原 G（HLA-G）基因和蛋白质表达改变以及甲基化谱之间的关系，这些妇女基于其健康的生活方式习惯。将 80 名年龄在 35 岁以下的妇女分为两组：1-POR 不使用塑料容器（n=40）和 2-POR 使用塑料容器（n=40）。通过定量 PCR 和 Western blot 技术检测 ICAM-1 和 HLA-G 基因和蛋白质表达。通过甲基化特异性 PCR 研究甲基化模式。采用高效液相色谱技术测定卵泡液中总 BPA，检测限为 1.14ng/ml。两组研究中 ICAM-1 和 HLA-G 基因表达存在差异。与第二组相比，第一组的 ICAM-1、HLA-G 基因和蛋白质表达上调（P<0.05）。而第一组的 DNA 甲基化状态较其他组降低（P<0.05）。与第二组相比，第一组卵泡液中 BPA 浓度较低（P<0.05）。与其他组相比，第一组的卵子质量和临床妊娠率明显更高（P<0.05）。POR 妇女中 ICAM-1 和 HLA-G 基因表达的改变可能与 BPA 浓度有关。因此，生活方式习惯也可能影响 POR 妇女卵丘细胞的甲基化模式和蛋白质水平。此外，生活方式习惯可以作为排卵、卵母细胞成熟、植入前和临床妊娠过程的标志物。
