人甲状旁腺激素相关蛋白片段-（1-34）在细胞化学生物测定中对远曲小管具有葡萄糖-6-磷酸脱氢酶活性。
Human parathyroid hormone related protein fragment-(1-34) had glucose-6-phosphate dehydrogenase activity on distal convoluted tubules in cytochemical bioassay.
作者信息
Nakai M, Fukase M, Sakaguchi K, Noda T, Fujii N, Fujita T
机构信息
Department of Medicine, Kobe University School of Medicine, Japan.
出版信息
Biochem Biophys Res Commun. 1988 Jul 15;154(1):146-50. doi: 10.1016/0006-291x(88)90662-6.
In the present study, the action of parathyroid hormone related protein (PTHrP) on glucose-6-phosphate dehydrogenase (G6PD) activity of the distal convoluted tubules was examined utilizing cytochemical bioassay (CBA). Recently full amino acid residues of human PTHrP (hPTHrP), one of the causative agents of HHM, was identified based on the cDNA clone using BEN cells. We synthesized hPTHrP-(1-34) and examined the effect of this protein on G6PD activities on the distal convoluted tubules, and compared its bioactivity to that of human parathyroid hormone (hPTH)-(1-84). hPTHrP-(1-34) stimulated G6PD activity in a log linear fashion with equivalent activity to that of hPTH-(1-84) on a molar basis. Conclusively, we found that PTHrP act on distal convoluted tubules similar to hPTH.
在本研究中，利用细胞化学生物测定法（CBA）检测了甲状旁腺激素相关蛋白（PTHrP）对远曲小管葡萄糖-6-磷酸脱氢酶（G6PD）活性的作用。最近，基于使用BEN细胞的cDNA克隆，确定了人类甲状旁腺激素相关蛋白（hPTHrP）（HHM的致病因子之一）的完整氨基酸残基。我们合成了hPTHrP-(1-34)，并检测了该蛋白对远曲小管G6PD活性的影响，并将其生物活性与人类甲状旁腺激素（hPTH）-(1-84)进行比较。hPTHrP-(1-34)以对数线性方式刺激G6PD活性，在摩尔基础上与hPTH-(1-84)具有同等活性。最终，我们发现PTHrP对远曲小管的作用与hPTH相似。
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