伴刀豆球蛋白A对心脏肌膜钠钙交换的刺激作用。

Stimulation of heart sarcolemmal Na+-Ca2+ exchange by concanavalin A.

作者信息

Makino N, Zhao D, Dhalla N S

机构信息

Division of Cardiovascular Sciences, St. Boniface General Hospital Research Centre, Winnipeg, Canada.

出版信息

Biochem Biophys Res Commun. 1988 Jul 15;154(1):245-51. doi: 10.1016/0006-291x(88)90676-6.

DOI:10.1016/0006-291x(88)90676-6

PMID:3395327

Abstract

The effects of Concanavalin A (Con A) on membrane Ca2+ translocation activities were examined by employing rat heart sarcolemmal preparations. Con A stimulated Na+-dependent Ca2+ uptake and ATP-dependent Ca2+ uptake activities in the sarcolemmal vesicles; maximal stimulation was seen at a concentration of 10 microgram/ml. These effects of Con A were blocked by alpha-metylmannoside. Sarcolemmal Na+-induced Ca2+ release was not affected by Con A. It is suggested that Con A-like substances may play a regulatory role in Ca2+-translocation processes of heart sarcolemma.

摘要

通过使用大鼠心肌肌膜制剂，研究了伴刀豆球蛋白A（Con A）对膜Ca2+转运活性的影响。Con A刺激了肌膜囊泡中Na+依赖性Ca2+摄取和ATP依赖性Ca2+摄取活性；在浓度为10微克/毫升时观察到最大刺激作用。Con A的这些作用被α-甲基甘露糖苷阻断。肌膜Na+诱导的Ca2+释放不受Con A影响。提示Con A样物质可能在心肌肌膜的Ca2+转运过程中起调节作用。