与嵌入型恶唑并吡啶咔唑鎓共价连接的α-异头体和β-异头体四胸苷酸的聚（rA）结合。结合参数的测定。
Poly(rA) binding of alpha-anomeric and beta-anomeric tetrathymidylates covalently linked to an intercalating oxazolopyridocarbazolium. Determination of the binding parameters.
作者信息
Gautier C, Bazile D, Paoletti J
机构信息
Laboratoire de Biochimie, INSERM U. 140, CNRS LA. 147, Institut Gustave-Roussy, Villejuif, France.
出版信息
Biochem Biophys Res Commun. 1988 Jul 15;154(1):252-7. doi: 10.1016/0006-291x(88)90677-8.
PMID:3395328
Abstract
We have investigated by means of absorbance measurements at 310 nm the binding of alpha-anomeric or beta-anomeric tetrathymidylates covalently substituted at their 3' end by an intercalating agent (oxazolopyridocarbazolium), to poly(rA). Taking into account the strong autoaggregation of the free ligands, we have derived the binding parameters corresponding to the [alpha] and the [beta] ligands. The affinity of the alpha-anomer for poly(rA) is higher than the affinity of the beta-anomer in accordance with the Tm studies conducted on such a system.
摘要
我们通过在310nm处进行吸光度测量，研究了在其3'端通过嵌入剂（恶唑并吡啶咔唑鎓）共价取代的α-异头或β-异头四胸苷酸与聚（rA）的结合。考虑到游离配体的强烈自聚集，我们得出了与α和β配体相对应的结合参数。根据对该系统进行的熔解温度研究，α-异头体对聚（rA）的亲和力高于β-异头体。
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引用本文的文献
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