Sinigaglia F, Bisio A, Torti M, Balduini C L, Bertolino G, Balduini C
Department of Biochemistry, University of Pavia, Italy.
Biochem Biophys Res Commun. 1988 Jul 15;154(1):258-64. doi: 10.1016/0006-291x(88)90678-x.
We studied the influence of the occupancy of the fibrinogen receptor (GP IIb-IIIa complex) on two early aspects of agonist induced platelet activation: the increase of the intracellular Ca2+ concentration and the cytoskeleton reorganization. A monoclonal antibody, a peptide containing the RGD sequence and fibrinogen purified from human plasma were used as GP IIb-IIIa ligands. The obtained results demonstrated that fibrinogen receptor occupancy inhibits Ca2+ movement and cytoskeleton reorganization caused by low thrombin concentration and ADP.
我们研究了纤维蛋白原受体（GP IIb-IIIa复合物）的占据情况对激动剂诱导的血小板活化的两个早期方面的影响：细胞内Ca2+浓度的升高和细胞骨架重组。一种单克隆抗体、一种含有RGD序列的肽以及从人血浆中纯化的纤维蛋白原被用作GP IIb-IIIa配体。所得结果表明，纤维蛋白原受体的占据会抑制低浓度凝血酶和ADP引起的Ca2+移动和细胞骨架重组。
