Vasdev S, Thompson P, Triggle C, Fernandez P, Bolli P, Ananthanarayanan V S
Disciplines of Medicine, Memorial University, St. John's, Newfoundland, Canada.
Biochem Biophys Res Commun. 1988 Jul 15;154(1):380-6. doi: 10.1016/0006-291x(88)90696-1.
Elevated intracellular free calcium concentration [Ca2+]i in vascular smooth muscle cells has been implicated in the pathophysiology of hypertension. Platelet [Ca2+]i was measured using the fluorescent indicator, Fura-2, in Dahl sensitive (DS) and resistant (DR) rats given high (8% NaCl) and low (0.4% NaCl) salt diets, as well as in the spontaneously hypertensive (SHR) and Wistar-Kyoto (WKY) rats. The aim of this study was to show whether [Ca2+]i is elevated in salt induced hypertension. Platelet [Ca2+]i and systolic blood pressure (SBP) were higher (p less than 0.001) in DS rats given a high than low salt diets. In contrast, no changes in platelet [Ca2+]i and SBP were observed in DR rats. In SHR, platelet [Ca2+]i and SBP were higher (p less than 0.001) than in the WKY rats. Platelet [Ca2+]i correlated with SBP in all groups of rats (r = 0.929; p less than 0.001, n = 38). The parallel increase in SBP and [Ca2+]i in the DS high salt rats and the SHR suggests that an increased [Ca2+]i is involved in the pathophysiology of hypertension in the two models which differ with respect to the pathogenesis of their hypertension. This increase in [Ca2+]i therefore seems to reflect an abnormality in [Ca2+]i handling in hypertension regardless of its cause.
血管平滑肌细胞内游离钙浓度[Ca2+]i升高与高血压的病理生理学有关。使用荧光指示剂Fura-2测量给予高盐（8%氯化钠）和低盐（0.4%氯化钠）饮食的Dahl敏感（DS）和抗性（DR）大鼠以及自发性高血压（SHR）和Wistar-Kyoto（WKY）大鼠的血小板[Ca2+]i。本研究的目的是表明在盐诱导的高血压中[Ca2+]i是否升高。给予高盐饮食的DS大鼠的血小板[Ca2+]i和收缩压（SBP）高于给予低盐饮食的大鼠（p<0.001）。相比之下，DR大鼠的血小板[Ca2+]i和SBP没有变化。在SHR中，血小板[Ca2+]i和SBP高于WKY大鼠（p<0.001）。所有组大鼠的血小板[Ca2+]i与SBP相关（r = 0.929；p<0.001，n = 38）。DS高盐大鼠和SHR中SBP和[Ca2+]i的平行升高表明，[Ca2+]i升高参与了这两种高血压发病机制不同的模型的高血压病理生理学。因此，这种[Ca2+]i的升高似乎反映了高血压中[Ca2+]i处理的异常，无论其原因如何。
