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缬草标准化提取物对Wistar大鼠细胞因子、抗体及迟发型超敏反应的免疫调节作用。

Immunomodulatory effects of standardized extract of val. on cytokines, antibody and delayed-type hypersensitivity response in Wistar rats.

作者信息

Nugraha Sony Eka, Laila Lia, Satria Denny

机构信息

Department of Pharmacology, Faculty of Pharmacy, Universitas Sumatera Utara, Indonesia.

Centre of Excellence for Chitosan and Advanced Materials, Universitas Sumatera Utara, Indonesia.

出版信息

Res Pharm Sci. 2020 Dec 30;16(1):16-25. doi: 10.4103/1735-5362.305185. eCollection 2021 Feb.

DOI:10.4103/1735-5362.305185
PMID:33953771
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8074802/
Abstract

BACKGROUND AND PURPOSE

Recently, we have highlighted the immunomodulatory activity of Val. on phagocytosis ability. The current study was conducted to determine the immunomodulatory effects of the standardized extract of rhizomes by and studies.

EXPERIMENTAL APPROACH

The extract was standardized according to a guideline for herbal preparation. The extract was investigated for its immunomodulatory effects on gene expression of cytokines, cytokines and antibody production as well as delayed-type hypersensitivity (DTH) response. The gene expression of cytokines on lipopolysaccharide-induced-RAW 264.7 cells was analysed by reverse transcription-polymerase chain reaction (RT-PCR) method. The effect of the extract on DTH response was investigated by the paw edema method, meanwhile the effects of the extract on antibody and cytokine production from normal and cyclophosphamide-induced infected rats were determined using an enzyme-linked immunosorbent assay (ELISA).

FINDINGS/RESULTS: The extract of demonstrated an inhibitory effect on gene expression of interleukin-1β (IL-1β), tumor necrosis factor-α, and IL-6 as compared to lipopolysaccharide-induced cells. The extract also depicted inhibitory activity on IL-4 production as compared to the negative control. Whereas, the DTH response and production of immunoglobulin G from both groups after treatment with extract were higher than those of negative control ( < 0.05).

CONCLUSION AND IMPLICATIONS

The results indicated that the extract has immunomodulatory effects, emphasizing its potential to be developed as immunotherapeutic agent.

摘要

背景与目的

最近，我们着重强调了缬氨酸对吞噬能力的免疫调节活性。本研究旨在通过[具体实验方法]确定[根茎名称]标准化提取物的免疫调节作用。

实验方法

根据草药制剂指南对[提取物名称]提取物进行标准化。研究该提取物对细胞因子基因表达、细胞因子和抗体产生以及迟发型超敏反应（DTH）的免疫调节作用。采用逆转录-聚合酶链反应（RT-PCR）法分析脂多糖诱导的RAW 264.7细胞中细胞因子的基因表达。通过爪肿胀法研究提取物对DTH反应的影响，同时使用酶联免疫吸附测定（ELISA）法测定提取物对正常和环磷酰胺诱导的[感染类型]感染大鼠抗体和细胞因子产生的影响。

研究结果

与脂多糖诱导的细胞相比，[提取物名称]提取物对白细胞介素-1β（IL-1β）、肿瘤坏死因子-α和IL-6的基因表达具有抑制作用。与阴性对照相比，该提取物对IL-4的产生也表现出抑制活性。然而，用[提取物名称]提取物处理后，两组的DTH反应和免疫球蛋白G的产生均高于阴性对照（P<0.05）。

结论与意义

结果表明，[提取物名称]提取物具有免疫调节作用，强调了其作为免疫治疗剂开发的潜力。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6310/8074802/5f392336f6b7/RPS-16-16-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6310/8074802/a9c8eba410f0/RPS-16-16-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6310/8074802/93f60f4b17b1/RPS-16-16-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6310/8074802/b264681f0cd8/RPS-16-16-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6310/8074802/5f392336f6b7/RPS-16-16-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6310/8074802/a9c8eba410f0/RPS-16-16-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6310/8074802/93f60f4b17b1/RPS-16-16-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6310/8074802/b264681f0cd8/RPS-16-16-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6310/8074802/5f392336f6b7/RPS-16-16-g004.jpg

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