Ahangarpour Akram, Alboghobeish Soheila, Oroojan Ali Akbar, Dehghani Mohammad Amin
Diabetes Research Center, Health Research Institute, Department of Physiology, Faculty of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.
Student Research Committee, Department of Pharmacology, School of Pharmacy, Ahvaz Jundishapur University of Medical Sciences, Ahvaz,Iran.
Vet Res Forum. 2021 Winter;12(1):77-85. doi: 10.30466/vrf.2019.94666.2279. Epub 2021 Mar 15.
Increasing applications of carbon nanotubes (CNTs) indicate the necessity to examine their toxicity. According to previous studies, CNTs caused oxidative stress that impaired β-cell functions and reduced insulin secretion. Our previous study indicated that single-walled carbon nanotubes (SWCNTs) could induce oxidative stress in pancreatic islets. However, there is no study on the effects of multi-walled carbon nanotubes (MWCNTs) on islets and β-cells. Therefore, the present study aims to evaluate effects of MWCNTs on the oxidative stress of islets and the protective effects of caffeic acid (CA) as an antioxidant. The effects of MWCNTs and CA on islets were investigated using MTT assay, reactive oxygen species (ROS), malondialdehyde (MDA), activities of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GSH-Px), the content of glutathione (GSH) and mitochondrial membrane potential (MMP) and insulin secretion measurements. The lower viability of islet cells was dose-dependent due to the exposure to MWCNTs according to the MTT assay. Further studies revealed that MWCNTs decreased insulin secretion and MMP, induced ROS creation, increased the MDA level, and decreased activities of SOD, GSH-Px, CAT, and content of GSH. Furthermore, the pretreatment of islets with CA returned the changes. These findings indicated that MWCNTs might induce the oxidative stress of pancreatic islets occurring diabetes and protective CA effects that were mediated by the augmentation of the antioxidant defense system of islets. Our research suggested the necessity of conducting further studies on effects of MWCNTs and CA on the diabetes.
碳纳米管（CNTs）应用的不断增加表明有必要研究其毒性。根据先前的研究，碳纳米管会引起氧化应激，损害β细胞功能并减少胰岛素分泌。我们之前的研究表明，单壁碳纳米管（SWCNTs）可诱导胰岛产生氧化应激。然而，尚无关于多壁碳纳米管（MWCNTs）对胰岛和β细胞影响的研究。因此，本研究旨在评估MWCNTs对胰岛氧化应激的影响以及抗氧化剂咖啡酸（CA）的保护作用。使用MTT法、活性氧（ROS）、丙二醛（MDA）、超氧化物歧化酶（SOD）、过氧化氢酶（CAT）、谷胱甘肽过氧化物酶（GSH-Px）活性、谷胱甘肽（GSH）含量、线粒体膜电位（MMP）以及胰岛素分泌测量等方法，研究了MWCNTs和CA对胰岛的影响。根据MTT法，由于暴露于MWCNTs，胰岛细胞活力降低呈剂量依赖性。进一步研究表明，MWCNTs降低胰岛素分泌和MMP，诱导ROS生成，增加MDA水平，并降低SOD、GSH-Px、CAT活性以及GSH含量。此外，用CA预处理胰岛可使这些变化恢复。这些发现表明，MWCNTs可能诱导胰岛氧化应激从而引发糖尿病，而CA的保护作用是通过增强胰岛抗氧化防御系统介导的。我们的研究表明有必要进一步研究MWCNTs和CA对糖尿病的影响。
