Department of Ultrasound, The First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, 310003, China.
CAS Key Laboratory of Quantitative Engineering Biology, Shenzhen Institute of Synthetic Biology, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen, 518055, China.
Biomater Sci. 2021 Jun 4;9(11):4127-4138. doi: 10.1039/d1bm00251a.
Ultrasound-activated microbubble destruction is a promising platform for gene delivery due to the low toxicity, non-invasiveness, and high specificity. However, the gene transfection efficiency is still low, especially for suspension cells. It is desirable to develop a universal gene delivery tool that overcomes the drawbacks existing in ultrasound-mediated methods. Here, we present a three-dimensional acoustic field-based conformal transfection (AFCT) system by designing a Sono-hole that can fit the three-dimensional acoustic field to maximally utilize the acoustic energy from bubble cavitation, thus greatly promoting the gene delivery efficiency. Surprisingly, compared with the traditional two-dimensional transfection system, the gene transfection efficiency of the AFCT system increased by more than 3 times, achieving nearly 30%. The parameters including acoustic pressure, duration, duty cycle, DNA concentrations, and bubble kinds were optimized to obtain higher gene transfection. In conclusion, our study provides an effective ultrasound-based gene delivery approach for gene transfection, especially for suspension-cultured cells.
超声激活微泡破坏因其低毒性、非侵入性和高特异性，是一种很有前途的基因传递平台。然而，基因转染效率仍然较低，特别是对于悬浮细胞。理想情况下，需要开发一种通用的基因传递工具，以克服超声介导方法存在的缺点。在这里，我们通过设计一个 Sono-hole 来呈现一个基于三维声场的适形转染（AFCT）系统，该 Sono-hole 可以适应三维声场，以最大限度地利用空化泡的声能，从而极大地提高基因传递效率。令人惊讶的是，与传统的二维转染系统相比，AFCT 系统的基因转染效率提高了 3 倍以上，达到近 30%。优化了声压、持续时间、占空比、DNA 浓度和气泡种类等参数，以获得更高的基因转染效率。总之，我们的研究为基因转染提供了一种有效的基于超声的基因传递方法，特别是对于悬浮培养的细胞。
