School of Physical and Mathematical Sciences, Nanyang Technological University, Singapore.
Chem Commun (Camb). 2021 May 7;57(37):4568-4571. doi: 10.1039/d0cc05890d. Epub 2021 Apr 12.
We generated a novel G-quadruplex (G4)-specific endonuclease by fusing a G4 recognition domain of the RHAU helicase with a cleavage domain of the Fok1 nuclease. The fusion protein can specifically bind a parallel G4 and cleave a double-stranded DNA (dsDNA) next to it. The new endonuclease could be used to detect a G4 in a long dsDNA, providing a useful tool for mapping the formation of G4s in the genome.
我们通过融合 RHAU 解旋酶的 G4 识别结构域和 Fok1 核酸内切酶的切割结构域，产生了一种新型的 G-四链体（G4）特异性内切酶。该融合蛋白可以特异性结合平行 G4 并切割其旁边的双链 DNA（dsDNA）。这种新的内切酶可用于检测长 dsDNA 中的 G4，为在基因组中绘制 G4 形成提供了有用的工具。
