比较 dl-蛋氨酸和 dl-蛋氨酸羟类似物钙盐与 l-蛋氨酸在仔猪氮保留方面的生物利用度。

Bioavailability of the dl-methionine and the calcium salt of dl-methionine hydroxy analog compared with l-methionine for nitrogen retention in starter pigs.

机构信息

Institute of Animal Nutrition, Sichuan Agricultural University, Chengdu 611130, China.

Nutrition & Care, Evonik (China) Co., Ltd. Beijing 100026, China.

出版信息

J Anim Sci. 2021 Jun 1;99(6). doi: 10.1093/jas/skab151.

DOI:10.1093/jas/skab151

PMID:33956968

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8202088/

Abstract

Two nitrogen balance studies were conducted to evaluate the relative bioavailability values (RBV) of dl-methionine (dl-Met) and dl-methionine hydroxy analog calcium salt (MHA-Ca) to l-methionine (l-Met) as Met sources fed to pigs. In experiment 1, 42 pigs were assigned to 7 treatments feeding with basal diet (BD) formulated to be deficient in Met (0.22% standardized ileal digestible basis) but adequate in other amino acids. Diets included (1) BD, (2) BD + 0.025% dl-Met, (3) BD + 0.050% dl-Met, (4) BD + 0.075% dl-Met, (5) BD + 0.025% l-Met, (6) BD + 0.050% l-Met, and (7) BD + 0.075% l-Met. Increasing levels of l-Met and dl-Met enhanced N retained (g/d) and N retention (% of intake) linearly (P < 0.01). Using a linear slope ratio procedure, a product-to-product RBV of dl-Met compared with l-Met was 94% (95% confidence limits: 65% to 123%) based on N retained expressed as g/d and 99% (95% confidence limits: 70% to 128%) for N retention expressed as % of intake. In experiment 2, 42 pigs were allotted to 7 treatments in another N-balance trial. Diets included (1) BD, (2) BD + 0.025% l-Met, (3) BD + 0.050% l-Met, (4) BD + 0.075% l-Met, (5) BD + 0.030% MHA-Ca, (6) BD + 0.060% MHA-Ca, and (7) BD + 0.089% MHA-Ca. An increase in dietary inclusion rates of l-Met increased (P < 0.01) N retained (g/d) linearly while increasing levels of MHA-Ca had no effects (P > 0.05) on N retained (g/d) and N retention (% of intake). Using linear slope-ratio regression, the RBV of MHA-Ca compared with l-Met was 70% (95% confidence limits: 59% to 81%) on a product-to-product basis or 83% on equimolar basis based on N retained expressed as g/d. Overall, the mean RBV of dl-Met to l-Met of 97% (95% confidence limits cover 100%) indicated that dl-Met and l-Met are equally bioavailable as Met sources in pigs. Compared with l-Met, the RBV of MHA-Ca was lower at 70% (95% confidence limits: 59% to 81%) on a product-to-product basis or 83% on equimolar basis in starter pigs.

摘要

进行了两项氮平衡研究，以评估 dl-蛋氨酸（dl-Met）和 dl-蛋氨酸羟基类似物钙盐（MHA-Ca）相对于 l-蛋氨酸（l-Met）作为蛋氨酸来源的相对生物利用价值（RBV），这些研究在猪中进行。在实验 1 中，将 42 头猪分配到 7 种处理中，用基础日粮（BD）进行饲养，该日粮的设计缺乏蛋氨酸（0.22% 标准回肠可消化基础），但其他氨基酸充足。日粮包括：（1）BD，（2）BD+0.025% dl-Met，（3）BD+0.050% dl-Met，（4）BD+0.075% dl-Met，（5）BD+0.025% l-Met，（6）BD+0.050% l-Met，和（7）BD+0.075% l-Met。增加 l-Met 和 dl-Met 的水平，线性提高了氮保留（g/d）和氮保留（%摄入量）（P<0.01）。使用线性斜率比程序，基于以 g/d 表示的氮保留，dl-Met 与 l-Met 的产物对产物 RBV 为 94%（95%置信区间：65%至 123%），基于以%摄入量表示的氮保留，RBV 为 99%（95%置信区间：70%至 128%）。在实验 2 中，在另一个氮平衡试验中，将 42 头猪分配到 7 种处理中。日粮包括：（1）BD，（2）BD+0.025% l-Met，（3）BD+0.050% l-Met，（4）BD+0.075% l-Met，（5）BD+0.030% MHA-Ca，（6）BD+0.060% MHA-Ca，和（7）BD+0.089% MHA-Ca。增加日粮中 l-Met 的添加率，线性提高了氮保留（g/d）（P<0.01），而增加 MHA-Ca 的水平对氮保留（g/d）和氮保留（%摄入量）没有影响（P>0.05）。使用线性斜率比回归，MHA-Ca 与 l-Met 的 RBV 为 70%（95%置信区间：59%至 81%），基于产物对产物，或基于以 g/d 表示的氮保留，为 83%，基于等摩尔基础。总体而言，dl-Met 对 l-Met 的平均 RBV 为 97%（95%置信区间涵盖 100%），表明 dl-Met 和 l-Met 作为猪的蛋氨酸来源具有同等的生物利用度。与 l-Met 相比，MHA-Ca 的 RBV 在仔猪中以产物对产物为基础为 70%（95%置信区间：59%至 81%）或基于等摩尔为基础为 83%时较低。