State Key Laboratory of Rice Biology, Zhejiang University, Hangzhou, China.
Key Laboratory of Molecular Biology of Crop Pathogens and Insects, Institute of Biotechnology, Zhejiang University, Hangzhou, China.
Nat Commun. 2021 May 6;12(1):2576. doi: 10.1038/s41467-021-22831-8.
Nitric oxide (NO) is a diffusible signaling molecule that modulates animal and plant immune responses. In addition, reactive nitrogen species derived from NO can display antimicrobial activities by reacting with microbial cellular components, leading to nitrosative stress (NS) in pathogens. Here, we identify FgAreB as a regulator of the NS response in Fusarium graminearum, a fungal pathogen of cereal crops. FgAreB serves as a pioneer transcription factor for recruitment of the chromatin-remodeling complex SWI/SNF at the promoters of genes involved in the NS response, thus promoting their transcription. FgAreB plays important roles in fungal infection and growth. Furthermore, we show that a transcription repressor (FgIxr1) competes with the SWI/SNF complex for FgAreB binding, and negatively regulates the NS response. NS, in turn, promotes the degradation of FgIxr1, thus enhancing the recruitment of the SWI/SNF complex by FgAreB.
一氧化氮（NO）是一种可扩散的信号分子，可调节动物和植物的免疫反应。此外，源自 NO 的反应性氮物种可以通过与微生物细胞成分反应显示出抗菌活性，从而导致病原体中的硝化应激（NS）。在这里，我们确定 FgAreB 是禾谷镰刀菌（一种谷物作物的真菌病原体）中 NS 反应的调节剂。FgAreB 作为先驱转录因子，可招募参与 NS 反应的基因启动子处的染色质重塑复合物 SWI/SNF，从而促进其转录。FgAreB 在真菌感染和生长中发挥重要作用。此外，我们表明转录阻遏物（FgIxr1）与 SWI/SNF 复合物竞争 FgAreB 结合，并负调控 NS 反应。反过来，NS 促进 FgIxr1 的降解，从而增强 FgAreB 对 SWI/SNF 复合物的募集。
