Laboratoire de Reproduction et Développement des Plantes, Université de Lyon, ENS de Lyon, UCBL, CNRS, INRAE, Allée d'Italie, France.
Plant Signal Behav. 2021 Aug 3;16(8):1921992. doi: 10.1080/15592324.2021.1921992. Epub 2021 May 7.
Following pollen deposition on the receptive surface of the stigma, pollen germinates a tube that carries male gametes toward the ovule where fertilization occurs. As soon as it emerges from the pollen grain, the pollen tube has to be properly guided through the pistil tissues so as to reach the ovule and ensure double fertilization. Chemical attractants, nutrients as well as receptor kinase-dependent signaling pathways have been implicated in this guidance. Recently, we showed in Arabidopsis that the microtubule severing enzyme KATANIN, by acting both on cortical microtubule (CMT) dynamics and cellulose microfibril (CMF) deposition, conferred particular mechanical properties to the papilla cell wall that act as active guidance factors. Here we confirm the importance of KATANIN and CMT orientation in pollen tube directionality by examining another mutant.
花粉沉积在柱头的接受表面后，花粉会萌发一个管，将雄性配子输送到发生受精的胚珠处。花粉管一旦从花粉粒中伸出，就必须通过雌蕊组织正确引导，以到达胚珠并确保双受精。化学引诱剂、营养物质以及受体激酶依赖性信号通路都与这种引导有关。最近，我们在拟南芥中表明，微管切割酶 KATANIN 通过作用于皮质微管（CMT）动力学和纤维素微纤维（CMF）沉积，赋予了乳突细胞壁特定的机械性能，作为主动导向因子。在这里，我们通过检查另一个突变体，证实了 KATANIN 和 CMT 取向在花粉管方向性中的重要性。
