Almeida-Paes Rodrigo, Figueiredo-Carvalho Maria Hg, da Silva Leandro Br, Gerfen Gary, S Araújo Glauber R de, Frases Susana, Zancopé-Oliveira Rosely M, Nosanchuk Joshua D
Laboratório de Micologia, Instituto Nacional de Infectologia Evandro Chagas, Fundação Oswaldo Cruz, Rio de Janeiro, RJ, Brazil.
Departments of Medicine (Division of Infectious Diseases) & Microbiology & Immunology, Albert Einstein College of Medicine, Bronx, NY, USA.
Future Microbiol. 2021 May;16(7):509-520. doi: 10.2217/fmb-2020-0228. Epub 2021 May 7.
Melanin has been linked to pathogenesis in several fungi. They often produce melanin-like pigments in the presence of L-dihydroxyphenylalanine (L-DOPA), but this is poorly studied in . was grown in minimal medium with or without L-DOPA supplementation and submitted to a chemical treatment with denaturant and hot acid. turned black when grown in the presence of L-DOPA, whereas cells grown without L-DOPA supplementation remained white. Biophysical properties demonstrated that the pigment was melanin. Melanized cells were effectively protected from azoles and amphotericin B, incubation at 42°C and macrophage killing. In the presence of L-DOPA, produces melanin, increases antifungal resistance and enhances host survival.
黑色素已与多种真菌的发病机制相关联。它们常在L-二羟基苯丙氨酸（L-DOPA）存在的情况下产生类黑色素色素，但对此研究甚少。在添加或不添加L-DOPA的基本培养基中培养，并进行变性剂和热酸的化学处理。在L-DOPA存在下生长时会变黑，而未添加L-DOPA生长的细胞仍为白色。生物物理特性表明该色素是黑色素。黑色素化的细胞能有效抵御唑类药物、两性霉素B、42°C孵育和巨噬细胞杀伤。在L-DOPA存在的情况下，会产生黑色素，增加抗真菌耐药性并提高宿主存活率。
