The Ritchie Centre, Hudson Institute of Medical Research, Clayton, VIC, Australia.
Obstetrics and Gynaecology, Monash University, Clayton, VIC, Australia.
Cell Tissue Res. 2021 Sep;385(3):803-815. doi: 10.1007/s00441-021-03453-4. Epub 2021 May 7.
Mesenchymal stem cells (MSCs) that meet the International Society for Cellular Therapy (ISCT) criteria are obtained from placental tissue by plastic adherence. Historically, no known single marker was available for isolating placental MSCs (pMSCs) from the decidua basalis. As the decidua basalis is derived from the regenerative endometrium, we hypothesised that SUSD2, an endometrial perivascular MSC marker, would purify maternal perivascular pMSC. Perivascular pMSCs were isolated from the maternal placenta using SUSD2 magnetic bead sorting and assessed for the colony-forming unit-fibroblasts (CFU-F), surface markers, and in vitro differentiation into mesodermal lineages. Multi-colour immunofluorescence was used to colocalise SUSD2 and α-SMA, a perivascular marker in the decidua basalis. Placental stromal cell suspensions comprised 5.1%SUSD2 cells. SUSD2 magnetic bead sorting of the placental stromal cells increased their purity approximately two-fold. SUSD2 pMSCs displayed greater CFU-F activity than SUSD2 stromal fibroblasts (pSFs). However, both SUSD2 pMSC and SUSD2 pSF underwent mesodermal differentiation in vitro, and both expressed the ISCT surface markers. Higher percentages of cultured SUSD2 pMSCs expressed the perivascular markers CD146, CD140b, and SUSD2 than SUSD2 pSFs. These findings suggest that SUSD2 is a single marker that enriches maternal pMSCs, suggesting they may originate from eMSC. Placental decidua basalis can be used as an alternative source of MSC for clinical translation in situations where there is no access to endometrial tissue.
间质干细胞（MSCs）符合国际细胞治疗学会（ISCT）标准，可通过塑料贴附从胎盘组织中获得。历史上，没有已知的单一标志物可用于从基底蜕膜中分离胎盘间充质干细胞（pMSCs）。由于基底蜕膜来源于再生子宫内膜，我们假设 SUSD2，一种子宫内膜血管周 MSC 标志物，将纯化母体血管周 pMSC。使用 SUSD2 磁珠分选从母体胎盘分离血管周 pMSCs，并评估其集落形成单位-成纤维细胞（CFU-F）、表面标志物和体外向中胚层谱系的分化。多色免疫荧光用于共定位 SUSD2 和 α-SMA，基底蜕膜中的血管周标志物。胎盘基质细胞悬液包含 5.1%的 SUSD2 细胞。SUSD2 磁珠分选胎盘基质细胞可将其纯度提高约两倍。SUSD2 pMSCs 的 CFU-F 活性大于 SUSD2 基质成纤维细胞（pSFs）。然而，SUSD2 pMSC 和 SUSD2 pSF 均可在体外进行中胚层分化，且均表达 ISCT 表面标志物。与 SUSD2 pSFs 相比，培养的 SUSD2 pMSCs 表达更高百分比的血管周标志物 CD146、CD140b 和 SUSD2。这些发现表明 SUSD2 是一种单一标志物，可富集母体 pMSCs，表明它们可能源自 eMSC。在无法获得子宫内膜组织的情况下，胎盘基底蜕膜可作为 MSC 的替代来源，用于临床转化。
