Jones A F, Winkles J W, Jennings P E, Florkowski C M, Lunec J, Barnett A H
Department of Biochemistry, Selly Oak Hospital, Birmingham, UK.
Diabetes Res. 1988 Feb;7(2):89-92.
Free radical medicated oxidative damage has previously been implicated in the pathogenesis of diabetic microangiopathy. Caeruloplasmin and transferrin act as antioxidants in serum by oxidizing and chelating ferrous iron which could otherwise act as a catalyst in free radical reactions. We have measured the serum anti-oxidant activity in 67 diabetics, 25 of whom had retinopathy and in 37 controls. Serum iron concentrations were normal in diabetics in comparison with controls (21.5 +/- 10 v 19.5 +/- 6.7 mumol/L) although transferrin and iron binding capacity were increased in the diabetics (3.7 +/- 0.8 v 2.9 +/- 0.9 g/L, p less than 0.001 and 69.9 +/- 10.8 v 59.0 +/- 14 mumol/L, p less than 0.002 respectively). Caeruloplasmin, measured by its ferroxidase activity, was increased in both male (46 +/- 11 v 36 +/- 9 mu/mL, p less than 0.002) and female diabetics (57 +/- 13 v 47 +/- 11 mu/mL, p less than 0.002). Ferritin was also increased in both male (124 +/- 113 v 44 +/- 38 ng/mL, p less than 0.001) and female diabetics (132 +/- 118 v 25 +/- 12 ng/mL, p less than 0.001). The presence of retinopathy, the degree of glycaemic control and duration of diabetes had no effect on these findings. We conclude that increased concentrations of iron-oxidizing and iron-binding proteins occur in diabetic serum, and that the increased serum antioxidant activity may be a response to oxidative stress.
自由基介导的氧化损伤先前已被认为与糖尿病微血管病变的发病机制有关。铜蓝蛋白和转铁蛋白通过氧化和螯合亚铁离子在血清中起到抗氧化剂的作用，否则亚铁离子可能会在自由基反应中充当催化剂。我们测量了67名糖尿病患者、其中25名患有视网膜病变患者以及37名对照者的血清抗氧化活性。与对照组相比，糖尿病患者的血清铁浓度正常（21.5±10对19.5±6.7μmol/L），尽管糖尿病患者的转铁蛋白和铁结合能力增加（3.7±0.8对2.9±0.9g/L，p<0.001；69.9±10.8对59.0±14μmol/L，p<0.002）。通过其亚铁氧化酶活性测量的铜蓝蛋白在男性（46±11对36±9μ/mL，p<0.002）和女性糖尿病患者（57±13对47±11μ/mL，p<0.002）中均升高。男性（124±113对44±38ng/mL，p<0.001）和女性糖尿病患者（132±118对25±12ng/mL，p<0.001）的铁蛋白也升高。视网膜病变的存在、血糖控制程度和糖尿病病程对这些结果没有影响。我们得出结论，糖尿病血清中铁氧化蛋白和铁结合蛋白的浓度增加，并且血清抗氧化活性的增加可能是对氧化应激的一种反应。
