Instituto de Virología Dr. J. M. Vanella, Facultad de Ciencias Médicas, Universidad Nacional de Córdoba, Córdoba, 5000, Argentina.
Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Argentina.
J Public Health (Oxf). 2021 Sep 22;43(3):e482-e486. doi: 10.1093/pubmed/fdab145.
To analyze the infectious extent of severe acute respiratory syndrome coronavirus 2 (SARS-COV-2) in different settings where prevention strategies are critical to limit infection spread, we evaluated SARS-COV-2 viability to guide public health policies regarding isolation criteria and infection control.
We attempted viral isolation in 82 nasopharyngeal swabs from 72 patients with confirmed SARS-COV-2 infection. Study population was divided into four groups: (i) Patients during the first week of symptoms; (ii) Patients with prolonged positive PCR; (iii) Healthcare workers from a hospital participating of an outbreak investigation, with SARS-COV-2 infection confirmed by reverse transcriptase polymerase chain reaction (RT-PCR) and (iv) Recipients of convalescent immune plasma (CIP).Vero Cl76 cell-line (ATCC CRL-587) was used in assays for virus isolation. Plasma samples of CIP recipients were also tested with plaque-reduction neutralization test.
We obtained infectious SARS-COV-2 isolates from 15/84 nasopharyngeal swabs. The virus could not be isolated from upper respiratory tract samples collected 10-day after onset of symptoms (AOS) in patients with mild-moderate disease.
The knowledge of the extent of SARS-CoV-2 infectivity AOS is relevant for effective prevention measures. This allows to discuss criteria for end isolation despite persistence of positive PCR and improve timing for hospital discharge with consequent availability of critical beds.
为了分析严重急性呼吸综合征冠状病毒 2 (SARS-CoV-2) 在不同环境中的感染范围，这些环境中的预防策略对于限制感染传播至关重要，我们评估了 SARS-CoV-2 的生存能力，以指导有关隔离标准和感染控制的公共卫生政策。
我们尝试从 72 名确诊 SARS-CoV-2 感染患者的 82 份鼻咽拭子中进行病毒分离。研究人群分为四组：(i) 症状出现后的第一周患者；(ii) PCR 持续阳性的患者；(iii) 参与暴发调查的医院的医护人员，SARS-CoV-2 感染通过逆转录聚合酶链反应 (RT-PCR) 确诊；(iv) 恢复期免疫血浆 (CIP) 的接受者。Vero Cl76 细胞系 (ATCC CRL-587) 用于病毒分离检测。CIP 接受者的血浆样本也通过蚀斑减少中和试验进行了检测。
我们从 84 份鼻咽拭子中的 15/84 份中获得了传染性 SARS-CoV-2 分离株。在症状出现后 10 天 (AOS) 收集的轻度-中度疾病患者的上呼吸道样本中，无法分离到病毒。
了解 SARS-CoV-2 感染的严重程度在 AOS 对于有效的预防措施非常重要。这使得可以讨论尽管持续存在阳性 PCR 但仍要结束隔离的标准，并改善医院出院时间，从而为重症监护病房提供更多可用床位。
