Gazi University, Faculty of Dentistry, Department of Oral and Maxillofacial Surgery, Ankara, Turkey.
Mugla Sitki Kocman University, Faculty of Science, Department of Biology, Mugla, Turkey.
Arch Oral Biol. 2021 Jul;127:105139. doi: 10.1016/j.archoralbio.2021.105139. Epub 2021 Apr 30.
Hypericum perforatum L also known as St. John's wort is known to have many beneficial properties for the organism including its antioxidant and anticancer activities. It is also known to have shown antiproliferative and cytotoxic effects against various cancer cell lines. The purpose of this study was to investigate the effects of Hypericum perforatum L on 7,12-dimethylbenz(a)anthracene-induced rat oral squamous cell carcinoma model.
The in vitro antioxidant properties of Hypericum perforatum L was determined and an extract was prepared. Thirty Wistar male rats were divided randomly into 4 groups (Control group, DMBA group, HP + DMBA group, HP group). The antioxidant defense mechanisms in tissue and blood samples were evaluated biochemically and immunohistochemically, the carcinomatous changes in connective tissue were investigated immunohistochemically and epithelial changes in the tissue samples were evaluated histopathologically.
The extract revealed inhibitory effects on some antioxidant enzymes (catalase, glutathione peroxidase). Immunohistochemical evaluations revealed no invasive changes in the connective tissue. Hypericum perforatum L demonstrated chemopreventive effects although it did not prevent carcinomatous changes altogether.
Hypericum perforatum L is a promising chemopreventive agent and further studies are needed in order to evaluate the full potential of this plant.
贯叶金丝桃（俗称贯叶连翘）被认为对生物体具有许多有益特性，包括抗氧化和抗癌活性。它还被证明对各种癌细胞系具有抗增殖和细胞毒性作用。本研究旨在探讨贯叶金丝桃对 7,12-二甲基苯并（a）蒽诱导的大鼠口腔鳞状细胞癌模型的影响。
体外测定了贯叶金丝桃的抗氧化特性，并制备了提取物。将 30 只雄性 Wistar 大鼠随机分为 4 组（对照组、DMBA 组、HP+DMBA 组、HP 组）。用生物化学和免疫组织化学方法评估组织和血液样本中的抗氧化防御机制，用免疫组织化学方法研究结缔组织中的癌前变化，用组织学方法评估组织样本中的上皮变化。
提取物对某些抗氧化酶（过氧化氢酶、谷胱甘肽过氧化物酶）显示出抑制作用。免疫组织化学评价显示结缔组织没有侵袭性变化。贯叶金丝桃具有化学预防作用，尽管它不能完全预防癌前变化。
贯叶金丝桃是一种很有前途的化学预防剂，需要进一步研究以评估这种植物的全部潜力。
