质谱分析可揭示参与脂质和药物转运的膜蛋白的结构和动态。
Mass spectrometry informs the structure and dynamics of membrane proteins involved in lipid and drug transport.
机构信息
Physical and Theoretical Chemistry Laboratory, Department of Chemistry, University of Oxford, Oxford, OX1 3QZ, UK; The Kavli Institute for Nanoscience Discovery, 3 South Parks Road, Oxford, OX1 3QU, UK.
Physical and Theoretical Chemistry Laboratory, Department of Chemistry, University of Oxford, Oxford, OX1 3QZ, UK; The Kavli Institute for Nanoscience Discovery, 3 South Parks Road, Oxford, OX1 3QU, UK.
出版信息
Curr Opin Struct Biol. 2021 Oct;70:53-60. doi: 10.1016/j.sbi.2021.03.014. Epub 2021 May 5.
Membrane proteins are important macromolecules that play crucial roles in many cellular and physiological processes. Over the past two decades, the use of mass spectrometry as a biophysical tool to characterise membrane proteins has grown steadily. By capturing these dynamic complexes in the gas phase, many unknown small molecule interactions have been revealed. One particular application of this research has been the focus on antibiotic resistance with considerable efforts being made to understand underlying mechanisms. Here we review recent advances in the application of mass spectrometry that have yielded both structural and dynamic information on the interactions of antibiotics with proteins involved in bacterial cell envelope biogenesis and drug efflux.
膜蛋白是重要的生物大分子，在许多细胞和生理过程中发挥着关键作用。在过去的二十年中，质谱作为一种生物物理工具来描述膜蛋白的应用一直在稳步增长。通过在气相中捕获这些动态复合物，揭示了许多未知的小分子相互作用。这项研究的一个特别应用是关注抗生素耐药性，人们做出了相当大的努力来了解潜在的机制。在这里，我们回顾了近年来质谱应用的进展，这些进展提供了抗生素与参与细菌细胞包膜生物发生和药物外排的蛋白质相互作用的结构和动态信息。
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