Key Laboratory of Water Science and Water Environment Recovery Engineering, Beijing University of Technology, Beijing 100123, China.
Key Laboratory of Water Science and Water Environment Recovery Engineering, Beijing University of Technology, Beijing 100123, China.
Sci Total Environ. 2021 Sep 10;786:147237. doi: 10.1016/j.scitotenv.2021.147237. Epub 2021 Apr 22.
The mixture of partial AGS and flocculent sludge in continuous flow reactors were operated with periodic famine (PF) strategy and continuous feast (CF) strategy to reveal the impact of the feast-famine strategies on cultivation of AGS and the dynamics of microbial communities. The experimental results showed that the mature AGS were cultivated with PF and CF strategy on the 31st and the 71st days respectively, which was the result of good extracellular polymeric substance (EPS) secretion with PF strategy. It could accelerate the formation of microbial aggregates due to the conditions of periodic famine. High-level denitrification with PF strategy via utilization of SMP was examined by EEM-PARAFAC on cycle test. The high-throughput pyrosequencing showed that the dominant bacteria with PF strategy involved functional bacteria of nutrient removal and EPS secreting bacteria, while the dominant bacteria were fast-growing heterotrophic organisms with CF strategy.
在连续流反应器中，将部分 AGS 和絮状污泥混合，分别采用周期性饥饿（PF）策略和连续饱食（CF）策略，以揭示饱食-饥饿策略对AGS 培养和微生物群落动态的影响。实验结果表明，分别在第 31 天和第 71 天，采用 PF 和 CF 策略培养成熟AGS，这是 PF 策略良好的胞外聚合物（EPS）分泌的结果。由于周期性饥饿的条件，它可以加速微生物聚集体的形成。通过循环试验的 EEM-PARAFAC 对 PF 策略的高水平脱氮进行了检验，发现其利用 SMP 进行。高通量焦磷酸测序表明，PF 策略的优势菌涉及营养物质去除的功能菌和 EPS 分泌菌，而 CF 策略的优势菌是快速生长的异养生物。
