Mitsuhashi N, Mizuno M, Miyagawa A, Kato J
Department of Obstetrics and Gynecology, Faculty of Medicine, University of Tokyo, Japan.
Endocrinol Jpn. 1988 Feb;35(1):131-4. doi: 10.1507/endocrj1954.35.131.
It was reported that unsaturated long chain fatty acids, such as arachidonic acid, oleic acid and docosahexaenoic acid inhibit the binding between progesterone and estrogen receptors and steroid hormones. Most of the long chain fatty acids are contained in phospholipids within the cells. The effect of phospholipids on the binding between R5020 and progesterone receptors was studied. Phosphatidyl ethanolamine and sphingomyelin had no effect on binding, but phosphatidyl inositol and phosphatidyl serine inhibited the binding 53% and 34% respectively. The effect of phosphatidyl inositol on the binding between R5020 and progesterone receptors was dose dependent. Scatchard analysis revealed that the addition of phospholipid markedly decreased the number of binding sites from 1398 fmol/mgp to 258 fmol/mgp, but the dissociation constant was little affected.
据报道，不饱和长链脂肪酸，如花生四烯酸、油酸和二十二碳六烯酸，会抑制孕酮与雌激素受体以及类固醇激素之间的结合。大多数长链脂肪酸存在于细胞内的磷脂中。研究了磷脂对R5020与孕酮受体结合的影响。磷脂酰乙醇胺和鞘磷脂对结合无影响，但磷脂酰肌醇和磷脂酰丝氨酸分别抑制结合53%和34%。磷脂酰肌醇对R5020与孕酮受体结合的影响呈剂量依赖性。Scatchard分析表明，添加磷脂显著降低了结合位点的数量，从1398 fmol/mgp降至258 fmol/mgp，但解离常数受影响较小。
