Hammer-Dedet Florence, Dupont Chloé, Evrevin Marine, Jumas-Bilak Estelle, Romano-Bertrand Sara
Hospital Hygiene and Infection Control Team, University Hospital of Montpellier, Montpellier, France.
Hospital Hygiene and Infection Control Team, University Hospital of Montpellier, Montpellier, France; HydroSciences Montpellier, IRD, CNRS, Montpellier University, Montpellier, France.
Infect Dis Now. 2021 Aug;51(5):488-491. doi: 10.1016/j.idnow.2021.04.003. Epub 2021 May 19.
Non-tuberculous mycobacteria (NTM) are opportunistic pathogens commonly colonizing hospital water systems, and may be responsible for healthcare-associated infections (HAI). Investigation of HAI and outbreaks caused by NTM necessitates water analyses. However, NTM are slow-growing bacteria within the mesophilic community present in water, and are difficult to detect. Prior to culture on specific media, their recovery usually requires decontamination and concentration steps. We assessed the effectiveness of filtration as regards the recovery of 7 NTM species in hospital water samples. We also compared the use of cetylpyridinium chloride (CPC) at different concentrations and Sodium Hydroxide (NaOH) 4% in decontamination of water samples with mesophilic bacteria. Our laboratory protocol showed that membrane filtration was suitable for concentration and recovery of NTM from water. Sample decontamination with CPC was more NTM-preservative than NaOH. A combination of CPC at 0.005% and filtration allowed detection of NTM at low concentrations, ranging from 3 to 98 CFU/100mL according to the NTM species.
非结核分枝杆菌（NTM）是常见于医院供水系统的机会性病原体，可能导致医疗保健相关感染（HAI）。对NTM引起的HAI和疫情进行调查需要对水进行分析。然而，NTM是水中嗜温菌群内生长缓慢的细菌，难以检测。在特定培养基上培养之前，其回收通常需要净化和浓缩步骤。我们评估了过滤对医院水样中7种NTM菌株回收的有效性。我们还比较了不同浓度的十六烷基氯化吡啶（CPC）和4%氢氧化钠（NaOH）在对含有嗜温细菌的水样进行净化时的效果。我们实验室的方案表明，膜过滤适用于从水中浓缩和回收NTM。用CPC对样品进行净化比用NaOH对NTM的保留性更好。0.005%的CPC与过滤相结合能够检测到低浓度的NTM，根据NTM菌株的不同，浓度范围为3至98 CFU/100mL。
