Gospodarowicz D, Ferrara N, Haaparanta T, Neufeld G
Cancer Research Institute, University of California Medical Center, San Francisco 94143.
Eur J Cell Biol. 1988 Apr;46(1):144-51.
The possible expression of fibroblast growth factor in cultured bovine vascular smooth muscle (VSM) cells derived from bovine adult aortic arch has been examined. VSM cells were found to express the basic fibroblast growth factor (bFGF) gene but not that of acidic fibroblast growth factor (aFGF), a related mitogen. Expression of bFGF was further examined. VSM cells contain 3.7 and 7.0 kilobase bFGF gene transcripts, which are translated into material closely related, if not identical, with bFGF when analyzed by its chromatographic behavior on heparin Sepharose, by immunoblot and by radioimmuno- and radioreceptor assays. Essentially all of the bFGF-like material is bioactive, i.e., it can stimulate the proliferation of capillary endothelial or VSM cells and the stimulation is blocked by anti-bFGF antibodies. Our results indicate that bFGF derived from VSM cells may act as a paracrine and autocrine growth factor for that cell type. Thus, bFGF could play an important role in vivo, in controlling the proliferation of VSM cells during embryonic growth of blood vessels or during atherosclerosis.
对源自成年牛主动脉弓的培养牛血管平滑肌（VSM）细胞中可能存在的成纤维细胞生长因子表达情况进行了研究。发现VSM细胞表达碱性成纤维细胞生长因子（bFGF）基因，但不表达相关有丝分裂原酸性成纤维细胞生长因子（aFGF）的基因。进一步研究了bFGF的表达情况。VSM细胞含有3.7和7.0千碱基的bFGF基因转录本，当通过其在肝素琼脂糖上的色谱行为、免疫印迹以及放射免疫和放射受体分析进行检测时，这些转录本被翻译成与bFGF密切相关（即便不是完全相同）的物质。基本上所有类似bFGF的物质都具有生物活性，也就是说，它能够刺激毛细血管内皮细胞或VSM细胞的增殖，并且这种刺激会被抗bFGF抗体阻断。我们的结果表明，源自VSM细胞的bFGF可能作为该细胞类型的旁分泌和自分泌生长因子。因此，bFGF在体内可能在控制血管胚胎生长期间或动脉粥样硬化期间VSM细胞的增殖方面发挥重要作用。
