School of Basic Medical Sciences, Xinxiang Medical University, Xinxiang 453003, China.
School of Basic Medical Sciences, Xinxiang Medical University, Xinxiang 453003, China.
Spectrochim Acta A Mol Biomol Spectrosc. 2021 Oct 5;259:119826. doi: 10.1016/j.saa.2021.119826. Epub 2021 Apr 20.
Cysteine (Cys) is an effective biomarker in life systems and is closely related to a variety of diseases, so developing a specific and efficient detection method for Cys is of great significance. To date, extensive work has been undertaken toward this goal. However, the differentiation of Cys from other biothiols still represents a challenge from an experimental point of view. Toward this end, a selective and sensitive red-emitting probe (TMN-NCS) with an isothiocyanate (ITC)-based structure was proposed in this paper. A large Stokes shift (210 nm) was observed upon addition of Cys to a solution of TMN-NCS. In addition, TMN-NCS showed low toxicity, a low detection limit (120 nM), and excellent cell permeability. The results suggested that TMN-NCS holds great promise for biological applications.
半胱氨酸（Cys）是生命体系中的一种有效生物标志物，与多种疾病密切相关，因此开发针对 Cys 的特异性和高效检测方法具有重要意义。迄今为止，已经开展了广泛的工作来实现这一目标。然而，从实验角度来看，Cys 与其他生物硫醇的区分仍然具有挑战性。为此，本文提出了一种基于异硫氰酸酯（ITC）结构的选择性和灵敏的红色发射探针（TMN-NCS）。向 TMN-NCS 溶液中加入 Cys 后，观察到较大的斯托克斯位移（210nm）。此外，TMN-NCS 具有低毒性、低检测限（120nM）和良好的细胞通透性。结果表明，TMN-NCS 在生物应用中具有广阔的应用前景。
