冷冻保存对大蒜（Allium sativum L.）植株生长、鳞茎特性及病毒减少的影响
Effect of Cryopreservation on Plant Growth, Bulb Characteristics, and Virus Reduction of Garlic (Allium sativum L.).
作者信息
Liu X, Mou S, Cheng Z
机构信息
College of Horticulture, Northwest A&F University, Yangling, Shaanxi, People's Republic of China.
College of Agriculture, Northwest A&F University, Yangling, Shaanxi, People's Republic of China.
出版信息
Cryo Letters. 2019 Nov-Dec;40(6):322-332.
BACKGROUND
Cryopreservation is a promising plant germplasm preservation technique that provides genetically stable plantlets.
OBJECTIVE
To investigate whether the cryopreservation protocol developed for garlic compromises the performance of the cryopreserved plants, compared to garlic plants grown in the field.
MATERIALS AND METHODS
The field performance of cryopreserved garlic plantlets under in vivo conditions was compared with garlic derived from the field. We assessed both net photosynthetic rate, bulb characteristics and the efficiency of cryotherapy-induced virus eradication.
RESULTS
The superiority of the morphological traits of cryopreserved garlic increased gradually with the growth of the garlic plant. Cryopreservation also improved the net photosynthetic rate, bulb diameter (by 13.4-18.2%), bulb weight (by 37.9-50.5%), and clove number per bulb (by 25.7-63.6%). The highest virus elimination rate due to cryopreservation was 75.0% for onion yellow dwarf virus (OYDV).
CONCLUSION
These findings suggest cryopreservation as a promising method for conservation and improvement of garlic.
背景
冷冻保存是一种很有前景的植物种质保存技术，可提供基因稳定的植株。
目的
与田间种植的大蒜植株相比，研究为大蒜开发的冷冻保存方案是否会影响冷冻保存植株的性能。
材料与方法
将冷冻保存的大蒜植株在体内条件下的田间性能与田间来源的大蒜进行比较。我们评估了净光合速率、鳞茎特征以及冷冻疗法诱导的病毒清除效率。
结果
冷冻保存大蒜的形态特征优势随着大蒜植株的生长而逐渐增加。冷冻保存还提高了净光合速率、鳞茎直径（提高了13.4 - 18.2%）、鳞茎重量（提高了37.9 - 50.5%）以及每鳞茎的蒜瓣数（提高了25.7 - 63.6%）。冷冻保存导致的洋葱黄矮病毒（OYDV）最高病毒清除率为75.0%。
结论
这些发现表明冷冻保存是一种用于大蒜保存和改良的有前景的方法。
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