Adzet T, Camarasa J, Escubedo E, Merlos M
Dep Farmacognosia y Farmacodinamia, Facultad de Farmacia, Universidad de Barcelona, Nucleo Universitario de Pedralbes, Espana.
Eur J Drug Metab Pharmacokinet. 1988 Jan-Mar;13(1):11-4. doi: 10.1007/BF03189921.
Caffeic acid, a natural product with pharmacological properties, such as DOPA-decarboxylase and 5-lipooxygenase inhibition, has been tested in vitro for its binding ratio to bovine serum albumin. This study was carried out by means of four analytical methods. Equilibrium dialysis has been proved to be the most reliable in determination of total binding sites, while acid precipitation has been evaluated as a model of irreversible binding. A binding ratio between 61 and 95% was observed, also varying according the caffeic acid and albumin concentration, pH and protein conformation. Binding constants were calculated by mathematical fitting to a hyperbolic plot. Other cinnamic acids non-o-diphenolic (ferulic acid, m- and p-coumaric acids) have been proved to have a binding ratio to bovine serum albumin of less than 10%.
咖啡酸是一种具有药理特性的天然产物，如抑制多巴脱羧酶和5-脂氧合酶，其与牛血清白蛋白的结合率已在体外进行了测试。本研究通过四种分析方法进行。平衡透析已被证明是测定总结合位点最可靠的方法，而酸沉淀已被评估为不可逆结合的模型。观察到的结合率在61%至95%之间，也会根据咖啡酸和白蛋白的浓度、pH值和蛋白质构象而变化。通过对双曲线图进行数学拟合来计算结合常数。已证明其他非邻二酚的肉桂酸（阿魏酸、间香豆酸和对香豆酸）与牛血清白蛋白的结合率小于10%。
