运动时肌肉 HbO 和 MbO 饱和度的近红外光谱（NIRS）。

Near-Infrared Spectroscopy (NIRS) of Muscle HbO and MbO Desaturation During Exercise.

机构信息

Department of Neurosurgery, University of New Mexico, Albuquerque, NM, USA.

出版信息

Adv Exp Med Biol. 2021;1269:83-86. doi: 10.1007/978-3-030-48238-1_13.

Abstract

Continuous noninvasive monitoring of muscle oxygenation has important clinical applications for muscle disorders such as compartmentation syndrome, fibromyalgia, deep vein thrombosis, malignant hyperthermia, and the assessment of training in athletic performance. NIRS has precisely such potential and has been used to detect deep venous thrombosis, evaluate athletic performance, and assess limb reperfusion and revascularization. The aim of this study was to examine the relationship between muscle hemoglobin oxygen (HbO) and myoglobin (MbO) desaturation using NIRS combined with venous blood sampling and HbO desaturation during forearm muscle exercise. Eleven normal subjects were studied, with informed consent and an IRB-approved protocol. A NIRS sensor (INVOS4100, Somanetics, Corp.) was applied on the volar aspect of the forearm. The subjects exercised their forearm by clenching and relaxing their fist while observing the oximeter and driving the reading to specified levels from 90% to 15% (minimum possible reading). Venous blood samples were withdrawn for measurement of blood gases and oxygen saturation (IL-Co-Oximeter). RSO (%) vs VO Sat showed a two-component HbO desaturation suggesting representation of venous HbO desaturation and perhaps myoglobin oxygen (MBO) desaturation. Subtraction of the linear venous HbO curve from the two-component curve suggests MbO desaturation at venous hemoglobin oxygen saturation of about 10-20%. Conclusions: The kinetics of desaturation during exercise revealed two components representing HbO and MbO deoxygenation. The data show that MbO represents approximately 40% of the NIRS signal and the balance or 60% to HbO.

摘要

连续无创监测肌肉氧合对于肌肉疾病如间隔综合征、纤维肌痛、深静脉血栓形成、恶性高热以及运动表现的评估具有重要的临床应用。NIRS 正是具有这种潜力，并已被用于检测深静脉血栓形成、评估运动表现以及评估肢体再灌注和再血管化。本研究旨在通过使用 NIRS 结合静脉采血来检测肌肉血红蛋白氧（HbO）和肌红蛋白（MbO）饱和度的关系，并在进行前臂肌肉运动时评估 HbO 饱和度。11 名正常受试者参与了这项研究，他们签署了知情同意书并遵循了 IRB 批准的方案。NIRS 传感器（INVOS4100，Somanetics，Corp.）应用于前臂的掌侧。受试者通过紧握和放松拳头来锻炼前臂，同时观察血氧计并将读数驱动至特定水平，从 90%降至 15%（最小可能读数）。抽取静脉血样进行血气和氧饱和度测量（IL-Co-Oximeter）。RSO（%）与 VO Sat 显示 HbO 饱和度的两成分下降，提示静脉 HbO 饱和度和可能的肌红蛋白氧（MBO）饱和度下降。从两成分曲线中减去线性静脉 HbO 曲线提示 MbO 饱和度约为静脉血红蛋白氧饱和度的 10-20%。结论：运动期间饱和度的动力学揭示了代表 HbO 和 MbO 去氧合的两个成分。数据表明 MbO 代表 NIRS 信号的约 40%，而其余 60%或平衡代表 HbO。