Fukuta K, Goto N, Inoue T, Imamura K, Mikami H, Onishi A
National Institute of Animal Health, Ibaraki, Japan.
Jikken Dobutsu. 1988 Apr;37(2):165-70. doi: 10.1538/expanim1978.37.2_165.
The incidence of the interfrontal bone in mice differs between strains, being high in C57BL/6 and low in BALB/c. In the present study, aggregation chimeras (C57BL----BALB) were examined to reveal whether or not genetically different cells interact in the morphogenesis of the interfrontal bone. In C57BL/6 mice, large interfrontal bones appeared in almost all animals, whereas in BALB/c and reciprocal F1 crosses (C57 BL x BALB, BALB x C57BL), large bones were seldom observed while tiny bones at the inside of the skull appeared at a low incidence. In contrast, chimeras frequently had large interfrontal bones, the size of which varied considerably. Based on the degree of chimerism as determined by coat color mosaicism and glucose phosphate isomerase (G PI) analysis of tissues, the chimeric mice containing a dominant population of C57BL cells had large interfrontal bones, while those with a predominance of BALB cells had no bone or had tiny ones. The results indicated that the appearance of the interfrontal bone corresponded with the population of the C57BL cells occupying the skeletal rudiment, and that there was no interaction between C57BL cells and BALB cells in the morphogenesis of the interfrontal bone.
小鼠额间骨的发生率因品系而异，在C57BL/6中较高，而在BALB/c中较低。在本研究中，对聚集嵌合体（C57BL----BALB）进行了检查，以揭示基因不同的细胞在额间骨形态发生过程中是否相互作用。在C57BL/6小鼠中，几乎所有动物都出现了较大的额间骨，而在BALB/c和 reciprocal F1杂交种（C57 BL×BALB，BALB×C57BL）中，很少观察到较大的骨头，而颅骨内部的小骨头出现的频率较低。相比之下，嵌合体经常有较大的额间骨，其大小差异很大。根据通过毛色嵌合现象和组织的葡萄糖磷酸异构酶（G PI）分析确定的嵌合程度，含有主要为C57BL细胞群体的嵌合小鼠有较大的额间骨，而主要为BALB细胞的小鼠则没有骨头或只有小骨头。结果表明，额间骨的出现与占据骨骼原基的C57BL细胞群体相对应，并且在额间骨的形态发生过程中，C57BL细胞和BALB细胞之间没有相互作用。
