Sidransky H, Verney E, Kurl R N, Razavi T
Department of Pathology, George Washington University Medical-Center, Washington, DC 20037.
Exp Mol Pathol. 1988 Aug;49(1):102-10. doi: 10.1016/0014-4800(88)90024-x.
The effects of the administration of tryptophan on toxic cirrhosis induced by intermittent carbon tetrachloride (CCl4) intoxication in the rat were investigated. Rats received CCl4 (0.45 ml/100 g body wt ip) twice weekly for 10-14 weeks. Tryptophan (30 mg/100 g body wt) by stomach tube was administered 1 hr before killing. Tryptophan improved hepatic polyribosomal aggregation and [14C]leucine incorporation into protein in vitro of control rats as well as long-term CCl4-treated rats that had developed toxic cirrhosis. However, the effects were more marked in control than in experimental rats. Tryptophan administration induced an increase in labeled nuclear RNA release in vitro and a decrease in labeled tryptophan binding to nuclear protein in vitro of livers of rats receiving long-term CCl4 and of control rats. The results indicate that the stimulatory effects of a single administration of tryptophan in toxic cirrhotic livers are similar to, but somewhat less than, those which occur in livers of normal, control rats.
研究了给予色氨酸对大鼠间歇性四氯化碳（CCl4）中毒诱导的中毒性肝硬化的影响。大鼠每周两次接受CCl4（0.45 ml/100 g体重，腹腔注射），持续10 - 14周。在处死前1小时通过胃管给予色氨酸（30 mg/100 g体重）。色氨酸改善了对照大鼠以及已发展为中毒性肝硬化的长期CCl4处理大鼠肝脏的多核糖体聚集和体外[14C]亮氨酸掺入蛋白质的情况。然而，对照大鼠中的作用比实验大鼠中更明显。给予色氨酸导致长期接受CCl4处理的大鼠和对照大鼠肝脏的体外标记核RNA释放增加，以及体外标记色氨酸与核蛋白的结合减少。结果表明，单次给予色氨酸对中毒性肝硬化肝脏的刺激作用与正常对照大鼠肝脏中的作用相似，但略小。
