Shimizu N, Kamel-Reid S, Zak R
Department of Medicine, University of Chicago, Illinois 60637.
Dev Biol. 1988 Aug;128(2):435-40. doi: 10.1016/0012-1606(88)90305-3.
The expression of actin genes in chicken pectoralis muscle denervated 1 week after hatching was examined 1-8 weeks after the operation by RNA blot hybridization using a generic actin cDNA probe and DNA probes specific for alpha-skeletal and alpha-cardiac actin genes. Total and alpha-skeletal actin mRNAs/microgram total RNA decreased to about half of the levels found in contralateral control muscle, while the expression of alpha-cardiac actin mRNA was up-regulated. Consequently, alpha-cardiac actin mRNA formed about 15% of the total actin mRNA as compared to less than 1% found in control muscle. The expression of actin genes in the denervated muscle was similar to that in the late embryonic muscle. These results suggest that innervation is required to show the expression pattern of striated muscle actin genes found in mature muscle.
利用通用肌动蛋白cDNA探针以及针对α-骨骼肌和α-心肌动蛋白基因的DNA探针，通过RNA印迹杂交法，对孵化后1周去神经支配的鸡胸大肌中肌动蛋白基因的表达进行了术后1 - 8周的检测。总的和α-骨骼肌肌动蛋白mRNA/微克总RNA降至对侧对照肌肉中所发现水平的约一半，而α-心肌动蛋白mRNA的表达上调。因此，与对照肌肉中不到1%相比，α-心肌动蛋白mRNA占总肌动蛋白mRNA的约15%。去神经支配肌肉中肌动蛋白基因的表达与胚胎后期肌肉中的相似。这些结果表明，需要神经支配才能展现出成熟肌肉中所见的横纹肌肌动蛋白基因的表达模式。
