Wang Xia, Ge Yansong, Shi Mingqi, Dai Hanhan, Liu Wei, Wang Peiyuan
Department of Pathology, Binzhou Medical University, Yantai, Shandong 264003, P.R. China.
Department of Radiology, Yantai Affiliated Hospital of Binzhou Medical University, Yantai, Shandong 264100, P.R. China.
Exp Ther Med. 2021 Jun;21(6):651. doi: 10.3892/etm.2021.10083. Epub 2021 Apr 19.
Protein kinase (PK) N1, also called PKC-related protein 1, participates in the proliferation, invasion and metastasis of various malignant tumors. However, the role of PKN1 in liver cancer remains to be elucidated. The present study investigated the expression of PKN1 using immunohistochemistry in surgical specimens from 36 patients and analyzed the correlation with VEGF, microvascular density (MVD), cell proliferation index (Ki67) and clinicopathological parameters. PKN1 was highly expressed in hepatocellular carcinoma (HCC) and was positively correlated with histological grading of HCC, Ki67 expression and MVD. PKN1 expression in moderately and poorly differentiated HCC was significantly higher compared with highly differentiated HCC. Expression of PKN1 was positively correlated with Ki67 and MVD, and Ki67 expression was positively correlated with MVD. The effects of PKN1 on proliferation, invasion and apoptosis of liver cancer cells were detected . Cell viability, migration and invasion were reduced and the apoptosis rate was significantly improved when PKN1 expression was silenced in liver cancer cells. Thus, PKN1 serves an important role in the development and progression of liver cancer. Inhibition of PKN1 activity may provide a promising therapeutic target for liver cancer.
蛋白激酶（PK）N1，也称为蛋白激酶C相关蛋白1，参与多种恶性肿瘤的增殖、侵袭和转移。然而，PKN1在肝癌中的作用仍有待阐明。本研究采用免疫组织化学方法检测了36例手术标本中PKN1的表达，并分析了其与血管内皮生长因子（VEGF）、微血管密度（MVD）、细胞增殖指数（Ki67）及临床病理参数的相关性。PKN1在肝细胞癌（HCC）中高表达，且与HCC的组织学分级、Ki67表达及MVD呈正相关。中低分化HCC中PKN1的表达明显高于高分化HCC。PKN1的表达与Ki67和MVD呈正相关，且Ki67表达与MVD呈正相关。检测了PKN1对肝癌细胞增殖、侵袭和凋亡的影响。当肝癌细胞中PKN1表达沉默时，细胞活力、迁移和侵袭能力降低，凋亡率显著提高。因此，PKN1在肝癌的发生发展中起重要作用。抑制PKN1活性可能为肝癌提供一个有前景的治疗靶点。
