Nithya P R, Manimegalai S, Nakkeeran S, Mohankumar S
Division of Agricultural Entomology, Regional Agricultural Research Station, Kerala Agricultural University, Palakkad, Kerala India.
Department of Agricultural Entomology, Tamil Nadu Agricultural University, Coimbatore, India.
3 Biotech. 2021 May;11(5):223. doi: 10.1007/s13205-021-02760-5. Epub 2021 Apr 18.
In the present investigation, hyperparasitic interaction between TM (MH590235) and was studied through scanning electron microscopy and chromatographic techniques. Dose-mortality responses showed an increase in mortality of larva with an increase in spore concentration. The LC value for isolate TM (MH590235) was 2.4 × 10 spores mL. The ditrophic interaction between and after 24 h revealed the adherence of conidia on stemmata, sensory setae, maxillary palpi and legs. After 48 h post-infection (hpi), germination of the conidia and appressorium formation was observed. Formation of hyphae and initiation of fungal networking was observed at 72 hpi. Complete ramification by mycelia and conidiogenesis of was observed over the mycosed cadaver after 168 hpi. Subsequently, typical sympodial conidiophores of bearing secondary spores were also observed. The metabolome profile of healthy larvae of revealed the presence of non-volatile organic compounds (NVOCs) like docosene, nonadecene, palmitic acid and heneicosane. However, the NVOC profile was different in the larvae hyperparasitized by The metabolites present in the infected cadaver were phthalate esters, hydroxyquebrachamine and lactones.
在本研究中，通过扫描电子显微镜和色谱技术研究了TM（MH590235）与[未提及的对象]之间的超寄生相互作用。剂量-死亡率反应表明，随着孢子浓度的增加，幼虫死亡率上升。分离株TM（MH590235）的LC值为2.4×10⁶个孢子/毫升。24小时后，[未提及的对象]与[未提及的对象]之间的双营养相互作用显示分生孢子附着在单眼、感觉刚毛、下颚须和腿上。感染后48小时（hpi），观察到分生孢子萌发和附着胞形成。在72 hpi时观察到菌丝形成和真菌网络的起始。在168 hpi后，在被真菌感染的尸体上观察到菌丝体的完全分支和[未提及的对象]的分生孢子形成。随后，还观察到带有次生孢子的典型合轴分生孢子梗。[未提及的对象]健康幼虫的代谢组谱显示存在如二十二碳烯、十九碳烯、棕榈酸和二十一烷等非挥发性有机化合物（NVOCs）。然而，被[未提及的对象]超寄生的[未提及的对象]幼虫的NVOC谱有所不同。感染尸体中存在的代谢产物是邻苯二甲酸酯、羟基去甲肾上腺素和内酯。
