Correa-Cuadros J P, Sáenz-Aponte A, Rodríguez-Bocanegra M X
Laboratorio de Control Biológico, Grupo de Biología de Plantas y Sistemas Productivos, Departamento de Biología, Facultad de Ciencias, Pontificia Universidad Javeriana, Bogotá, D.C. Colombia.
Unidad de Investigaciones Agropecuarias, Departamento de Microbiología, Facultad de Ciencias, Pontificia Universidad Javeriana, Bogotá, D.C. Colombia.
Springerplus. 2016 Dec 1;5(1):2068. doi: 10.1186/s40064-016-3745-5. eCollection 2016.
The diamondback moth () is a major pest of broccoli crops in Colombia. To control , we evaluated the interaction of Bb9205 and Ma9236 with HNI0100 and its bacterial symbiont HNI0100. We used antagonism and disk diffusion assays with fungal extracts to test the interaction between symbiotic bacterium and fungi. inhibited the growth of and up to 40% by the secretion of secondary metabolites, whereas fungal extracts did not inhibit ; this explains the in vivo interactions of these biological control agents. To test the interaction between fungi and nematodes, we first inoculated the fungi followed by the nematodes on different days (0, 2, 4, and 6). We identified the type of interaction using the formula by Nishimatsu and Jackson (J Econ Entomol 91:410-418, 1998) and established that on days 0, 2 and 4 there was an antagonistic interaction, while a synergistic interaction occurred on day 6. Therefore, the use of the interaction between HNI0100 with Ma9236 and Bb9205 is an innovative alternative for the control of .
小菜蛾是哥伦比亚西兰花作物的主要害虫。为了防治小菜蛾，我们评估了Bb9205和Ma9236与HNI0100及其细菌共生体HNI0100之间的相互作用。我们使用拮抗作用和用真菌提取物进行的纸片扩散试验来测试共生细菌与真菌之间的相互作用。HNI0100通过分泌次生代谢产物对小菜蛾和Ma9236的生长抑制高达40%，而真菌提取物对小菜蛾没有抑制作用；这解释了这些生物防治剂在体内的相互作用。为了测试真菌与线虫之间的相互作用，我们首先在不同天数（0、2、4和6天）接种真菌，然后接种线虫。我们使用Nishimatsu和Jackson（《经济昆虫学杂志》91:410 - 418，1998年）的公式确定相互作用的类型，并确定在第0、2和4天存在拮抗相互作用，而在第6天发生协同相互作用。因此，利用HNI0100与Ma9236和Bb9205之间的相互作用是防治小菜蛾的一种创新方法。
