Deepak Venkataraman, Sundar William Arputha, Pandian Sureshbabu Ram Kumar, Sivasubramaniam Shiva D, Hariharan Nellaiah, Sundar Krishnan
Department of Biotechnology, Kalasalingam Academy of Research and Education, Krishnankoil, 626126 Tamilnadu India.
School of Human Sciences, College of Life and Natural Sciences, University of Derby, Kedleston Road, Derby, DE22 1GB UK.
3 Biotech. 2021 May;11(5):225. doi: 10.1007/s13205-021-02784-x. Epub 2021 Apr 19.
The aim of the current study is to ascertain the anticancer activity of exopolysaccharides (EPS) from probiotic in the 1, 2-dimethyl hydrazine (DMH)-induced colon cancer rat model and to determine the antioxidant status. Rats were divided into five groups of six animals each. Group I served as control, group II served as cancer control (DMH alone administered), group III as standard drug control (5-FU along with DMH) and group IV and V received EPS in two doses (200 mg/kg body weight and 400 mg/kg body weight along with DMH). EPS administration was found to reduce the number of polyps formed (Group IV-8.25 ± 1.258 and Group V-8.50 ± 1.732 vs Group II-14.50 ± 2.380) and to increase the levels of antioxidant enzymes viz. Superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPx) and antioxidants like vitamin C (Vit. C), reduced glutathione (GSH) which was found to be reduced in colon cancer control rats. The status of lipid peroxidation (LPO) was also evaluated. All the values which were affected by the supplementation of DMH were brought to near normal levels by the treatment with EPS. The well-preserved histology of colon and the biochemical evaluation also show that EPS could be a potential agent for the prevention and treatment of colon cancer.
本研究的目的是确定益生菌胞外多糖（EPS）在1,2-二甲基肼（DMH）诱导的结肠癌大鼠模型中的抗癌活性，并测定抗氧化状态。将大鼠分为五组，每组六只动物。第一组作为对照组，第二组作为癌症对照组（仅给予DMH），第三组作为标准药物对照组（5-氟尿嘧啶与DMH联合使用），第四组和第五组接受两种剂量的EPS（200mg/kg体重和400mg/kg体重，与DMH联合使用）。发现给予EPS可减少息肉形成的数量（第四组-8.25±1.258和第五组-8.50±1.732， vs 第二组-14.50±2.380），并提高抗氧化酶即超氧化物歧化酶（SOD）、过氧化氢酶（CAT）和谷胱甘肽过氧化物酶（GPx）的水平以及抗氧化剂如维生素C（Vit.C）、还原型谷胱甘肽（GSH）的水平，这些在结肠癌对照大鼠中被发现有所降低。还评估了脂质过氧化（LPO）的状态。所有受DMH补充影响的值通过EPS治疗恢复到接近正常水平。结肠保存良好的组织学和生化评估也表明，EPS可能是预防和治疗结肠癌的潜在药物。
