Meneely P M, Nordstrom K D
Fred Hutchinson Cancer Research Center, Seattle, Washington 98104.
Genetics. 1988 Jun;119(2):365-75. doi: 10.1093/genetics/119.2.365.
X chromosome duplications have been used previously to vary the dose of specific regions of the X chromosome to study dosage compensation and sex determination in Caenorhabditis elegans. We show here that duplications suppress and X-linked hypomorphic mutation and elevate the level of activity of an X-linked enzyme, although these two genes are located in a region of the X chromosome that is not duplicated. The effects do not depend on the region of the X chromosome duplicated and is stronger in strains with two doses of a duplication than in strains with one dose. This is evidence for a general elevation of X-linked gene expression in strains carrying X-chromosome duplications, consistent with the hypothesis that the duplications titrate a repressor acting on many X-linked genes.
先前曾利用X染色体重复来改变秀丽隐杆线虫X染色体特定区域的剂量，以研究剂量补偿和性别决定。我们在此表明，重复会抑制X连锁的亚效突变，并提高X连锁酶的活性水平，尽管这两个基因位于X染色体未重复的区域。这些效应不依赖于X染色体重复的区域，并且在具有两份重复的菌株中比在具有一份重复的菌株中更强。这证明了携带X染色体重复的菌株中X连锁基因表达普遍升高，这与重复滴定作用于许多X连锁基因的阻遏物这一假设一致。
