Department of Physiology, Inner Mongolia Medical University, Hohhot, Inner Mongolia 010110, China.
Department of Laboratory, Baotou Central Hospital, Baotou city, Inner Mongolia 014040, China.
Biomed Res Int. 2021 Apr 20;2021:2043415. doi: 10.1155/2021/2043415. eCollection 2021.
The protective effect of aspirin against myocardial hypertrophy (MH) was studied. Model rats of pressure overload MH were prepared by abdominal aortic coarctation. Rats were randomly divided into the sham group ( = 9), MH model group ( = 9), and MH+aspirin group ( = 9), which was, respectively, divided into the 4-week group and 8-week group according to the time of intragastric administration. Arterial blood pressure and left ventricular mass index (LVMI) were measured. Changes in myocardial tissue structure were observed by HE staining, Masson staining, and reticular fiber staining. Cardiomyocyte apoptosis was detected by TUNEL assay. The levels of TNF-, IL-10, TXA2, and PGI2 in myocardium and plasma were detected by ELISA. The arterial blood pressure in the MH model group was significantly higher than that in the 4- and 8-week sham groups, but that in the MH+aspirin group was significantly lower than that in the MH model group. At 4 and 8 weeks, the LVWI in the MH model group was significantly higher than that in the sham group, but it was significantly reduced after aspirin treatment. The myocardial cell hypertrophy was obvious, collagen fibers were proliferated, and reticular fibers were reduced in the 4- and 8-week MH model groups. Compared with the MH model groups, myocardial cells in the MH+aspirin groups were significantly reduced, the collagen fiber content was significantly reduced, and the reticular fiber content was increased. The apoptotic cardiomyocytes in the 4- and 8-week MH model groups were obviously increased. The apoptosis of myocardial cells in the MH+aspirin groups was obviously decreased. The TNF- levels in the myocardial tissue of the 4- and 8-week MH model groups were significantly increased, while those of the MH+aspirin groups were significantly decreased. There was no significant change in the IL-10 level or PGI2 level at 4 weeks. At 8 weeks, the PGI2 level was significantly decreased in the MH model group while significantly increased in the MH+aspirin group. The TXA2 levels were significantly increased in the 4- and 8-week MH model groups and those in the 4- and 8-week MH+aspirin groups were significantly lower. Aspirin has an anti-inflammatory effect, can effectively reduce the expression of inflammatory factors, inhibit myocardial apoptosis, and has a certain protective effect against MH.
本研究旨在探讨阿司匹林对心肌肥厚（MH）的保护作用。采用腹主动脉缩窄法制备压力超负荷 MH 模型大鼠，随机分为假手术组（n=9）、MH 模型组（n=9）、MH+阿司匹林组（n=9），分别于灌胃后 4 周、8 周时分为 4 周组和 8 周组。测量各组大鼠动脉血压、左心室质量指数（LVMI）。HE 染色、Masson 染色和网状纤维染色观察心肌组织形态学变化，TUNEL 法检测心肌细胞凋亡，ELISA 法检测心肌和血浆中 TNF-、IL-10、TXA2、PGI2 水平。结果显示，与 4 周、8 周假手术组比较，MH 模型组大鼠动脉血压明显升高，LVMI 明显增加（P<0.05）；与 MH 模型组比较，MH+阿司匹林组大鼠动脉血压明显降低，LVMI 明显减小（P<0.05）。4 周、8 周时，MH 模型组大鼠心肌细胞肥大明显，胶原纤维增生，网状纤维减少；与 MH 模型组比较，MH+阿司匹林组大鼠心肌细胞明显减少，胶原纤维含量减少，网状纤维含量增加。4 周、8 周时，MH 模型组大鼠心肌细胞凋亡明显增加，MH+阿司匹林组大鼠心肌细胞凋亡明显减少。4 周、8 周时，MH 模型组大鼠心肌组织 TNF-水平明显升高，IL-10 水平无明显变化，PGI2 水平明显降低；与 MH 模型组比较，MH+阿司匹林组大鼠心肌组织 TNF-水平明显降低，PGI2 水平明显升高。4 周时，各组大鼠 TXA2 水平无明显变化；8 周时，MH 模型组大鼠 TXA2 水平明显升高，MH+阿司匹林组大鼠 TXA2 水平明显降低。综上，阿司匹林具有抗炎作用，能有效降低炎症因子表达，抑制心肌细胞凋亡，对 MH 具有一定的保护作用。
