小鼠β-葡萄糖醛酸酶mRNA的完整核苷酸序列及其推导的多肽序列。

The complete nucleotide sequence of murine beta-glucuronidase mRNA and its deduced polypeptide.

作者信息

Gallagher P M, D'Amore M A, Lund S D, Ganschow R E

机构信息

Division of Basic Science Research, Children's Hospital Research Foundation, Cincinnati, Ohio 45229.

出版信息

Genomics. 1988 Apr;2(3):215-9. doi: 10.1016/0888-7543(88)90005-5.

DOI:10.1016/0888-7543(88)90005-5

PMID:3397060

Abstract

The complete nucleotide sequence of murine beta-glucuronidase (GUS) mRNA has been compiled from three overlapping cloned cDNAs and a single GUS-specific genomic clone. The sequence is composed of 2455 nucleotides, exclusive of the poly(A) tail. The 5' and 3' untranslated regions contain 12 and 499 bases, respectively, with the open reading frame encoding a polypeptide of 648 amino acids (74.2 kDa), including a 22 amino acid signal sequence. The nucleotide and deduced amino acid sequences of murine GUS are compared to those published for rat and human GUS and the results are presented. Murine GUS also shares amino acid sequence identity with Escherichia coli GUS and beta-galactosidase. The complete sequences of murine GUS mRNA and its deduced polypeptide provide a basis from which to study the mechanisms responsible for the well-characterized variation in GUS expression among inbred mouse strains.

摘要

小鼠β-葡萄糖醛酸酶（GUS）mRNA的完整核苷酸序列是由三个重叠的克隆cDNA和一个单一的GUS特异性基因组克隆汇编而成。该序列由2455个核苷酸组成，不包括聚腺苷酸尾。5'和3'非翻译区分别包含12个和499个碱基，开放阅读框编码一个648个氨基酸（74.2 kDa）的多肽，包括一个22个氨基酸的信号序列。将小鼠GUS的核苷酸和推导的氨基酸序列与已发表的大鼠和人类GUS的序列进行比较，并给出结果。小鼠GUS与大肠杆菌GUS和β-半乳糖苷酶也具有氨基酸序列同一性。小鼠GUS mRNA及其推导多肽的完整序列为研究近交系小鼠品系中GUS表达特征性变异的机制提供了基础。