Department of Ophthalmology, Centre hospitalier de l'Université de Montréal (CHUM), 1051 Sanguinet, D.01.2273, Montreal, Quebec, H2X 3E4, Canada; Department of Experimental Surgery, Faculty of Medicine, McGill University, 3655 Promenade Sir William Osler, Montreal, Quebec, H3G 0B1, Canada.
Department of Ophthalmology, Centre hospitalier de l'Université de Montréal (CHUM), 1051 Sanguinet, D.01.2273, Montreal, Quebec, H2X 3E4, Canada.
Exp Eye Res. 2021 Jul;208:108615. doi: 10.1016/j.exer.2021.108615. Epub 2021 May 7.
Animal models of the Boston keratoprosthesis type 1 (KPro) are needed to study glaucoma damage after KPro implantation to control for confounding comorbidities common in human KPro recipients. The purpose of this study was to determine the feasibility of establishing a reproducible mouse model of glaucoma after KPro surgery, specifically that of a miniaturized mouse model of KPro (mKPro). In the present study, a total of 20 corneas of donor C57BL/6 mice (n = 10) were implanted in one eye of each recipient BALB/C mouse (n = 20), assembled as part of the mKPro, either with or without intraoperative lensectomy. Main feasibility outcomes consisted in incidence rates of loss of tone, capsule nicking, and lens extrusion, as well as acquisition of posterior segment optical coherence tomography (OCT) images. With lensectomy (n = 10), loss of ocular tone and retinal detachment occurred in 100% of mice. Without lensectomy (n = 10), capsule nicking and opening, as well as lens extrusion, occurred in 80% of mice. Causes of these complications included the large proportion of intraocular volume occupied by the lens, the shallow anterior chamber, and thus the lack of available intraocular volume to implant the KPro if the lens remains present. Successful mouse KPro surgery may require a great deal of practice to be useful as a reproducible model. Animal KPro models ought to be pursued further by research teams in future studies.
需要建立波士顿角膜假体 1 型（KPro）的动物模型，以研究 KPro 植入后青光眼损伤，以控制人类 KPro 接受者常见的混杂合并症。本研究的目的是确定在 KPro 手术后建立青光眼可重复小鼠模型的可行性，特别是微型小鼠 KPro（mKPro）模型。在本研究中，总共 20 个供体 C57BL/6 小鼠（n=10）的角膜被植入到每个受体 BALB/C 小鼠（n=20）的一只眼睛中，作为 mKPro 的一部分组装，术中是否进行晶状体切除术。主要可行性结果包括眼压丧失、囊袋划痕和晶状体挤出的发生率，以及获得后节光学相干断层扫描（OCT）图像的情况。在进行晶状体切除术（n=10）的情况下，100%的小鼠出现眼压丧失和视网膜脱离。在未进行晶状体切除术（n=10）的情况下，80%的小鼠出现囊袋划痕和裂开，以及晶状体挤出。这些并发症的原因包括晶状体占据的眼内容积比例大、前房浅，因此如果晶状体仍然存在，植入 KPro 可利用的眼内容积有限。成功的小鼠 KPro 手术可能需要大量实践才能成为可重复的模型。动物 KPro 模型应该在未来的研究中由研究团队进一步探索。
