Institute of Health and Medical Technology, Hefei Institutes of Physical Science, Chinese Academy of Sciences, Hefei 230031, China; University of Science and Technology of China, Hefei 230026, China.
Institute of Health and Medical Technology, Hefei Institutes of Physical Science, Chinese Academy of Sciences, Hefei 230031, China.
Spectrochim Acta A Mol Biomol Spectrosc. 2021 Oct 5;259:119883. doi: 10.1016/j.saa.2021.119883. Epub 2021 Apr 30.
The sensitive detection and identification of toxicants in oily matrices have suffered from difficulty in poisoning incidents, therefore it is necessary to develop the rapid and efficient analytical method to realize the on-site screening and analyzing. In this report, the surface-enhanced Raman spectroscopy (SERS) method was used to detect paraquat and diquat poisons in various oily matrix coupled with solvent extraction. The solvent extraction not only remove interfering impurities of oily substrates, but also can enrich and separate the poisons from oily matrix. It was demonstrated that the ethanol as the extractant was suitable for the rapid separation of poisons such as paraquat (PQ) and diquat (DQ) in oily matrix (soy sauce, pasta sauce, sesame oil, chili oil). Moreover, combined with a handheld Raman spectrometer, the entire detection process was completed within 8 min with the level of 10 ppb PQ and 100 ppb DQ. Furthermore, double-blind experiments verify the reliability of this method. The results demonstrate that this rapid and convenient method could be used for the effective enrichment and sensitive detection of poisons in several oily matrix and has the grate potential application in emergency response and public safety.
在油基质中对毒物进行灵敏检测和识别一直是中毒事件的难点，因此有必要开发快速有效的分析方法，以实现现场筛选和分析。在本报告中，表面增强拉曼光谱（SERS）法与溶剂萃取相结合，用于检测各种油基质中的百草枯和敌草快毒物。溶剂萃取不仅可以去除油基质中干扰杂质，还可以从油基质中浓缩和分离毒物。结果表明，乙醇作为萃取剂适用于快速分离油基质（酱油、番茄酱、芝麻油、辣椒油）中的百草枯（PQ）和敌草快（DQ）等毒物。此外，结合手持式拉曼光谱仪，整个检测过程在 8 分钟内完成，PQ 的检出限为 10 ppb，DQ 的检出限为 100 ppb。此外，双盲实验验证了该方法的可靠性。结果表明，该快速便捷的方法可用于几种油基质中毒物的有效富集和灵敏检测，在应急响应和公共安全方面具有很大的应用潜力。
