Skov Sørensen U B, Blom J, Birch-Andersen A, Henrichsen J
World Health Organization Collaborating Centre for Reference and Research on Pneumococci, Statens Seruminstitut, Copenhagen, Denmark.
Infect Immun. 1988 Aug;56(8):1890-6. doi: 10.1128/iai.56.8.1890-1896.1988.
The localization of pneumococcal capsular and cell wall antigens was examined by immunoelectron microscopy. C polysaccharide (C-Ps), a common component of all pneumococci, was uniformly distributed on both the inside and outside of the cell walls. The thickness of the C-Ps varied with the strain. Encapsulated strains were covered by varied amounts of capsular polysaccharide concealing the C-Ps of the bacteria so as to render it inaccessible to anti-C-Ps antibodies. In addition to C-Ps, protein antigens were demonstrable on the surface of nonencapsulated pneumococci. The proteins were not masked by the C-Ps layer. An extra layer on the cell walls was conspicuous on electron micrographs of both rough and encapsulated pneumococci. The nature of this extra layer has not been disclosed. F antigen, another common antigen of pneumococci, was uniformly distributed on the surface of the plasma membranes. During the course of the experimental work a reproducible method of gold labeling immunoglobulins was developed.
通过免疫电子显微镜检查肺炎球菌荚膜和细胞壁抗原的定位。C多糖（C-Ps）是所有肺炎球菌的共同成分，均匀分布在细胞壁的内外。C-Ps的厚度因菌株而异。有荚膜的菌株被不同量的荚膜多糖覆盖，掩盖了细菌的C-Ps，从而使抗C-Ps抗体无法接近。除C-Ps外，在无荚膜肺炎球菌表面可检测到蛋白质抗原。这些蛋白质未被C-Ps层掩盖。在粗糙型和有荚膜肺炎球菌的电子显微照片上，细胞壁上的额外一层很明显。这一额外层的性质尚未揭示。F抗原是肺炎球菌的另一种常见抗原，均匀分布在质膜表面。在实验过程中，开发了一种可重复的金标记免疫球蛋白方法。
