Science for Life Laboratory, KTH - Royal Institute of Technology, Stockholm, Sweden.
Department of Molecular and Clinical Medicine, University of Gothenburg, The Wallenberg Laboratory, Sahlgrenska University Hospital, Gothenburg, Sweden.
Elife. 2021 May 11;10:e66921. doi: 10.7554/eLife.66921.
Myocardial infarction (MI) promotes a range of systemic effects, many of which are unknown. Here, we investigated the alterations associated with MI progression in heart and other metabolically active tissues (liver, skeletal muscle, and adipose) in a mouse model of MI (induced by ligating the left ascending coronary artery) and sham-operated mice. We performed a genome-wide transcriptomic analysis on tissue samples obtained 6- and 24 hr post MI or sham operation. By generating tissue-specific biological networks, we observed: (1) dysregulation in multiple biological processes (including immune system, mitochondrial dysfunction, fatty-acid beta-oxidation, and RNA and protein processing) across multiple tissues post MI and (2) tissue-specific dysregulation in biological processes in liver and heart post MI. Finally, we validated our findings in two independent MI cohorts. Overall, our integrative analysis highlighted both common and specific biological responses to MI across a range of metabolically active tissues.
心肌梗死（MI）会引发一系列全身效应，其中许多效应尚不清楚。在这里，我们研究了 MI 进展相关的改变，该模型是通过结扎左冠状动脉升支构建的，研究对象包括心肌、其他代谢活跃组织（肝脏、骨骼肌和脂肪组织）以及假手术组小鼠。我们对 MI 后 6 小时和 24 小时以及假手术组获得的组织样本进行了全基因组转录组分析。通过构建组织特异性生物网络，我们观察到：（1）MI 后多个组织中多种生物学过程（包括免疫系统、线粒体功能障碍、脂肪酸β氧化以及 RNA 和蛋白质加工）的失调；（2）MI 后肝脏和心脏中生物学过程的组织特异性失调。最后，我们在两个独立的 MI 队列中验证了我们的发现。总的来说，我们的综合分析突出了 MI 在多种代谢活跃组织中的共同和特异性生物学反应。
