Division of Animal Sciences, Gynecology, and Women's Health, University of Missouri, Columbia, Missouri, USA.
Department of Obstetrics, Gynecology, and Women's Health, University of Missouri, Columbia, Missouri, USA.
Mol Reprod Dev. 2021 Jul;88(7):482-489. doi: 10.1002/mrd.23473. Epub 2021 May 10.
Glands of the uterus are essential for the establishment of pregnancy in mice and their products regulate embryo implantation and stromal cell decidualization critical for pregnancy establishment. Forkhead box A2 (FOXA2) is expressed specifically in the glands and a critical regulator of their differentiation, development and function. Progesterone and FOXA2 regulate members of a serine proteinase gene family (Prss28 and Prss29). Here, CRISPR-Cas9 genome-editing was used to create mice with a heterozygous or homozygous deletion of Prss28 or/and Prss29 to determine their biological roles in uterine function. Female mice lacking Prss28 and Prss29 or both developed normally and were fertile without alterations in uterine histoarchitecture, uterine gland number, or and gene expression. Thus, Prss28 and Prss29 are dispensable for female fertility and do not impact endometrial gland development or uterine function mice.
子宫腺体对于小鼠妊娠的建立至关重要，其产物调节胚胎着床和基质细胞蜕膜化，这对于妊娠的建立至关重要。叉头框蛋白 A2（FOXA2）特异性表达于腺体中，是其分化、发育和功能的关键调节因子。孕激素和 FOXA2 调节丝氨酸蛋白酶基因家族（Prss28 和 Prss29）的成员。在这里，使用 CRISPR-Cas9 基因组编辑技术创建了杂合或纯合缺失 Prss28 或/和 Prss29 的小鼠，以确定它们在子宫功能中的生物学作用。缺乏 Prss28 和 Prss29 或两者的雌性小鼠正常发育且具有生育能力，子宫组织学结构、子宫腺体数量或基因表达均未发生改变。因此，Prss28 和 Prss29 对于雌性生育能力不是必需的，并且不影响子宫内膜腺体的发育或小鼠的子宫功能。
