酰胺对脂鲤（Prochilodus lineatus）精液冷冻保存的影响。
Effect of Amide on Semen Cryopreservation of Curimba (Prochilodus lineatus).
机构信息
Nucleus of Teaching and Research in Animal Reproduction, Federal University of Pelotas, Pelotas, Brazil.
出版信息
Cryo Letters. 2020 Jan-Feb;41(1):1-5.
BACKGROUND
The low molecular weight and high cellular permeability of amides make them suitable for use as penetrative cryoprotectants for sperm cells.
OBJECTIVE
This study aims to evaluate the effect of dimethylformamide (DMF) and dimethylacetamide (DMA) on sperm cryopreservation of Curimba (Prochilodus lineatus).
MATERIALS AND METHODS
Semen samples were diluted in media containing cryoprotectants [DMF, DMA and dimethyl sulfoxide (DMSO)]. Parameters of motility, membrane integrity, DNA integrity, mitochondrial functionality, viability and fertility were assessed upon thawing.
RESULTS
As compared to the 10% DMSO, DMA at 5% and DMF at 2% obtained the best results for the integrity of membrane, DNA and mitochondria; the motility parameters were best in the 2% and 5% DMF treatments. The best fertilization rates were demonstrated in 2%, 5%, and 8% DMF treatment groups.
CONCLUSION
DMF at 2%, 5%, and 8% provided the best results for both in vitro and in vivo assessments, and can efficiently cryopreserve semen of Prochilodus lineatus.
背景
酰胺类物质的低分子量和高细胞通透性使其适合用作精子细胞的渗透保护剂。
目的
本研究旨在评估二甲基甲酰胺（DMF）和二甲基乙酰胺（DMA）对脂鲤科（Prochilodus lineatus）精子冷冻保存的影响。
材料和方法
精液样本在含有冷冻保护剂（DMF、DMA 和二甲基亚砜（DMSO））的介质中稀释。解冻后评估运动性、膜完整性、DNA 完整性、线粒体功能、活力和生育力等参数。
结果
与 10％DMSO 相比，5％DMA 和 2％DMF 对膜、DNA 和线粒体的完整性获得最佳结果；2％和 5％DMF 处理的运动参数最佳。在 2％、5％和 8％DMF 处理组中显示出最佳的受精率。
结论
2％、5％和 8％DMF 在体外和体内评估中均提供最佳结果，可有效冷冻保存脂鲤科（Prochilodus lineatus）的精液。
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