印度水牛精液质量参数与脂质过氧化和抗氧化特性的关系受冷冻保存的影响。
Effect of Cryopreservation on Semen Quality Parameters in Relation to Lipid Peroxidation and Antioxidant Profile in Indian Buffalo.
机构信息
Division of Animal Reproduction, ICAR-Indian Veterinary Research Institute, Uttar Pradesh, India.
Department of Veterinary Gynaecology and Obstetrics, Lala Lajpat Rai University of Veterinary and Animal Sciences, Haryana, India.
出版信息
Cryo Letters. 2021 Jan-Feb;42(1):33-38.
BACKGROUND
Lipid peroxidation (LPO) due to oxidative stress leads to structural and functional changes in spermatozoa.
OBJECTIVE
To evaluate any association of various seminal characteristics at the pre- and post-cryopreservation stages with LPO and total antioxidant capacity (TAC) in Murrah buffalo semen samples.
MATERIALS AND METHODS
Sixty-five ejaculates from seven bulls were processed for cryopreservation in liquid nitrogen.
RESULTS
Only 31 (47.7%) samples were found satisfactory for inclusion in the further artificial insemination. A strong negative correlation was observed between LPO and individual progressive motility, TAC, viability, plasma membrane integrity as well as acrosome integrity of fresh spermatozoa. At the post-thaw stage, post-thaw motility, viability, plasma membrane integrity and acrosome integrity had strong positive correlation with TAC.
CONCLUSION
The effort to minimize LPO and enhance TAC shall play a pivotal role in improving buffalo semen quality upon cryopreservation.
背景
氧化应激导致的脂质过氧化（LPO）会导致精子的结构和功能发生变化。
目的
评估牛冷冻精液样本在冷冻保存前后的各种精液特性与 LPO 和总抗氧化能力（TAC）之间的关联。
材料与方法
对 7 头公牛的 65 份精液进行了液氮冷冻处理。
结果
只有 31 份（47.7%）样本被认为足够好，可用于进一步的人工授精。LPO 与新鲜精子的个体前向运动能力、TAC、活力、质膜完整性和顶体完整性呈强烈负相关。在解冻后阶段，解冻后活力、活力、质膜完整性和顶体完整性与 TAC 呈强正相关。
结论
努力减少 LPO 和提高 TAC 将在改善水牛精液冷冻保存质量方面发挥关键作用。
Zotero 插件