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谷胱甘肽生物合成酶的测定。

Assay of the enzymes of glutathione biosynthesis.

机构信息

Department of Chemistry and Biochemistry, Texas Woman's University, Denton, TX, 76204, United States.

出版信息

Anal Biochem. 2022 May 1;644:114218. doi: 10.1016/j.ab.2021.114218. Epub 2021 May 8.

DOI:10.1016/j.ab.2021.114218
PMID:33974889
Abstract

This article is dedicated to the late long-time Editor-in-Chief of Analytical Biochemistry, William Jakoby. As a graduate student, I remember reading many articles in Analytical Biochemistry and Methods in Enzymology, both volumes that Bill edited. I first met him as a graduate student presenting at the American Society of Biochemistry (and Molecular Biology) meetings. My Ph.D. advisor, Alton Meister, would bring over well-known biochemists and introduce me as Dr. Anderson, leaving me a bit tongue-tied being that I was still actually a humble graduate student! I next met Bill at my first Analytical Biochemistry Executive Editors meeting in San Diego when he was Editor-in-Chief Emeritus; I felt honored to be on the same board with him and serving the journal to which he had brought to prominence. His eyes were piercing and he was so sharp; his knowledge was both broad and deep. Since much of the large body of Bill's research was on glutathione S-transferases, my article focuses on the assay of the enzymes that synthesize glutathione, a substrate for glutathione S-transferases.

摘要

本文是献给已故的《分析生物化学》长期主编威廉·雅可比(William Jakoby)的。在读研究生时,我记得读过许多《分析生物化学》和《酶学方法》这两卷的文章,这两本书都是比尔编辑的。我第一次见到他是在作为研究生参加美国生物化学学会(和分子生物学)会议时。我的博士导师 Alton Meister 会带来一些著名的生化学家,并介绍我为 Anderson 博士,这让我有点结结巴巴,因为我实际上还是一个谦逊的研究生!我在圣地亚哥的第一次《分析生物化学》执行编辑会议上再次见到了比尔,当时他是名誉主编;我很荣幸能与他在同一个委员会任职,并为他曾担任主编的期刊服务。他目光敏锐,思维敏捷;他的知识既广泛又深刻。由于比尔的大部分研究都集中在谷胱甘肽 S-转移酶上,所以我的文章重点介绍了合成谷胱甘肽的酶的测定方法,谷胱甘肽是谷胱甘肽 S-转移酶的底物。

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