Baláz S, Kuchár A, Drevojánek J, Adamcová J, Vrbanová A
Department of Biochemical Technology, Slovak Polytechnic University, Bratislava, Czechoslovakia.
J Biochem Biophys Methods. 1988 May;16(1):75-85. doi: 10.1016/0165-022x(88)90105-4.
A chromatographic method for the determination of association constants of rapidly dissociable complexes is described and applied to quantification of liposome/saline partition coefficients using gel chromatography. The approach allows for estimation of the free solute concentration in the sample by simple manual processing of the intact right-hand part of the solute peak deformed due to gradual diffusion of the accumulated solute from the liposomes along the separation column. Validity of the procedure was confirmed by both reasonable agreement with equilibrium dialysis data and model-based deconvolution of the distorted peak into its two components corresponding to initially unbound compound and to that escaped from the liposomes during the separation process.
描述了一种用于测定快速解离复合物缔合常数的色谱方法，并将其应用于使用凝胶色谱法定量脂质体/盐水分配系数。该方法通过对由于累积溶质从脂质体沿分离柱逐渐扩散而变形的溶质峰的完整右侧部分进行简单手动处理，从而估计样品中的游离溶质浓度。该程序的有效性通过与平衡透析数据的合理一致性以及将扭曲峰基于模型解卷积为对应于最初未结合化合物和在分离过程中从脂质体中逸出的化合物的两个组分得到了证实。
