Department of Chemistry, Faculty of Science, Assiut University, 71516 Assiut, Egypt.
Department of Chemistry, Faculty of Science, Assiut University, 71516 Assiut, Egypt.
Spectrochim Acta A Mol Biomol Spectrosc. 2021 Oct 5;259:119858. doi: 10.1016/j.saa.2021.119858. Epub 2021 Apr 19.
Highly simple, sensitive and selective method is developed for the spectrophotometric determination of Metronidazole (MDZ) antibacterial drug either in pure form or in pharmaceutical formulations. This method is based on reduction of potassium permanganate by Metronidazole drug in sodium hydroxide solution to give green manganate ion which recorded at 610 nm. The method produced linear responses in the concentration range 4.28 - 59.91 µg mL with limit of detection (LOD) and limit of quantification (LOQ) 0.21 and 0.69 µg mL for Metronidazole drug respectively. The apparent molar absorptivity is 0.865 × 10 L mol cm, Sandell sensitivity is 0.019 µg cm and correlation coefficient is 0.951. The method is highly reproducible and has been applied to a wide variety of pharmaceutical formulations and the results compare favourably with those of official methods.
开发了一种高度简单、灵敏和选择性的分光光度法，用于测定甲硝唑（MDZ）抗菌药物的纯品或制剂中的甲硝唑药物。该方法基于甲硝唑药物在氢氧化钠溶液中还原高锰酸钾生成绿色的高锰酸根离子，在 610nm 处记录。该方法在 4.28-59.91µg mL 的浓度范围内产生线性响应，甲硝唑药物的检出限（LOD）和定量限（LOQ）分别为 0.21 和 0.69µg mL。甲硝唑药物的表观摩尔吸光系数为 0.865×10 L mol cm，桑德尔灵敏度为 0.019µg cm，相关系数为 0.951。该方法重现性好，已应用于多种药物制剂，结果与官方方法相比令人满意。
