Okazaki K, Nishikawa S, Sakano H
Department of Microbiology and Immunology, University of California, Berkeley 94720.
J Immunol. 1988 Aug 15;141(4):1348-52.
We have analyzed Ig gene rearrangement in the immunodeficient mutant mouse, CB-17 scid. Bone marrow stem cells from scid mice were cultured in the in vitro culture system of Whitlock and Witte. Ig gene rearrangement in the scid cells was studied by DNA cloning. Seven DNA clones of Ig H chain JH and DH regions were analyzed by DNA sequencing, and all the clones contained a failure in D-J joining. In the rearranged structure, both DH and JH coding sequences are either partly or completely deleted. Molecular mechanisms causing the aberrant DNA rearrangement are discussed.
我们分析了免疫缺陷突变小鼠CB-17 scid中的Ig基因重排。从scid小鼠中获取的骨髓干细胞在Whitlock和Witte的体外培养系统中培养。通过DNA克隆研究scid细胞中的Ig基因重排。对7个Ig重链JH和DH区域的DNA克隆进行DNA测序分析，所有克隆均存在D-J连接失败的情况。在重排结构中，DH和JH编码序列部分或完全缺失。文中讨论了导致异常DNA重排的分子机制。
